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Chiasma Counting in Maize Male Meiocytes

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Barley Transformation by Particle Bombardment Using Callus

Author: Sung-Yong Kim, date: 06/05/2020, view: 208, Q&A: 0

The delivery of desired construct into plant cells relies mainly on Agrobacterium-mediated transformation. However, Agrobacterium transformation is still very challenging for barley as only one genotype is amendable to ...

Toluidine Blue O Staining of Paraffin-Sectioned Maize Tissue

Authors: Josh Strable, Jeffrey R. Yen, Michael J. Scanlon and Anne W. Sylvester, date: 05/05/2020, view: 185, Q&A: 0

Histology incorporates microtechnique, the preparation of living material for observation and microscopy to study the anatomy of cells, tissues and organs. Interpreting histological patterns in a specimen may help to correlate form and function at ...

Chiasma Counting in Maize Male Meiocytes

Authors: Gaganpreet K. Sidhu and Wojciech P. Pawlowski, date: 05/05/2020, view: 280, Q&A: 0

This protocol combines a classical chromosome spreading technique with 3-dimensional image reconstruction for visualization of bivalent configurations during meiosis to aid chiasma counting.

Immunolabeling of Maize Meiocytes

Authors: Mateusz Zelkowski and Wojciech P. Pawlowski, date: 04/05/2020, view: 339, Q&A: 0

This protocol describes a quick method for immunolocalization of meiotic proteins in maize. It includes a fixation step that allows for long-term storage of material and provides good preservation of chromatin structure.

In vitro Conditions for Dark Growth and Analysis of Maize Seedlings

Authors: Jeffrey Farrow, Marschal A. Bellinger and Carolyn G. Rasmussen, date: 03/20/2020, view: 396, Q&A: 0

Maize is an important model organism for understanding plant traits essential for proper growth and germination. One type of growth, skotomorphogenesis, occurs in the absence of light. Seedlings grown in the absence of light exhibit dramatic ...

Using Seed Chipping to Genotype Maize Kernels

Authors: Alison M. Mills, Lindy A. Allsman, Sareen Leon and Carolyn G. Rasmussen, date: 03/20/2020, view: 568, Q&A: 0

Zea mays (maize) is an important model organism for studying monocot growth and development. Genotyping maize in the greenhouse or field can be time consuming and costly. Here, we describe a method to remove or chip a small amount of the ...

Transient Transformation of Maize BMS Suspension Cells via Particle Bombardment

Authors: Liang-Zi Zhou and Thomas Dresselhaus, date: 12/05/2019, view: 721, Q&A: 0

Maize is one of the most important crop species and serves also as a model plant for grass research. A major bottleneck in maize research is stable transformation, which is both time and cost consuming, but also a technical challenge for most labs ...

A Simple Protocol for Informative Visualization of Enriched Gene Ontology Terms

Authors: Titouan Bonnot, Morgane B. Gillard and Dawn H. Nagel, date: 11/20/2019, view: 2491, Q&A: 1

In genome-scale datasets, Gene Ontology (GO) enrichment is a common analysis to highlight functions over-represented or under-represented in a subset of differentially expressed genes to elucidate the biological significance of the results. However, ...

Isolation and Efficient Maize Protoplast Transformation

Authors: Lina Gomez-Cano, Fan Yang and Erich Grotewold, date: 08/20/2019, view: 1683, Q&A: 0

Establishing plant gene function and the study of gene expression regulation often require that genes (or gene variants) are introduced into plants and their activity somehow assayed. Some plants (e.g., Arabidopsis) are easily ...

Quantifying MAMP-induced Production of Reactive Oxygen Species in Sorghum and Maize

Authors: Rozalynne Samira, Xinye Zhang, Jennifer Kimball, Yaya Cui, Gary Stacey and Peter J. Balint-Kurti, date: 07/20/2019, view: 1198, Q&A: 0

Plants have the ability to recognize microbe-associated molecular patterns (MAMPs) and mount a defense response. The level of the MAMP response can vary depending on genetic and environmental factors. The most commonly studied MAMPs are flg22, a ...

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