Home
About
For Authors
Submit Protocol
Request a Protocol
My bio page
Reset the password
Home
About
For Authors
Submit Protocol
Request a Protocol
A high-quality database for basic life science protocols
Search
Plant Science
Latest Protocol Video
Mating and Tetrad Dissection in
Chlamydomonas
Categories
By Date
By View
Maize Protein Extraction for Different Downstream Applications
Author:
María Jazmín Abraham-Juárez
,
date:
05/20/2019,
view:
36,
Q&A:
0
Sample preparation is a key step for making plant proteomics analyses reproducible. The large amount of organic compounds, complex polysaccharides, proteases and other cellular components in vascular plants interfere with the analysis of proteins ...
Hormone Treatments during Maize Vegetative and Reproductive Development
Authors:
María Jazmín Abraham-Juárez
and
Harry Klein
,
date:
05/20/2019,
view:
43,
Q&A:
0
Plant hormones are a group of organic natural compounds that can influence physiological processes when present at low concentrations. Plant biologists frequently use exogenous hormone application to modify plant physiology or to rescue mutants with ...
Efficient Assembly of Large Multiplex CRISPR/Cas9 Guide Arrays for Maize Genome Editing
Authors:
Jarrett Man
and
Madelaine E. Bartlett
,
date:
05/05/2019,
view:
101,
Q&A:
0
CRISPR/Cas9-based genome editing in maize is an effective tool, and researchers commonly wish to target multiple genes simultaneously. Transformation of maize is currently expensive and tedious, so researchers are incentivized to build vectors that ...
Staining Maize Epidermal Leaf Peels with Toluidine Blue O
Authors:
Marschal A. Bellinger
,
Sukhmani K. Sidhu
and
Carolyn G. Rasmussen
,
date:
04/20/2019,
view:
169,
Q&A:
0
Maize is an important model organism for understanding cell patterning and development. The regular patterning of maize leaf epidermal cells has previously been characterized using the dye toluidine blue O (TBO). The polychromatic dye TBO ...
Glue Impressions of Maize Leaves and Their Use in Classifying Mutants
Authors:
Lindy A. Allsman
,
Raquel N. Dieffenbacher
and
Carolyn G. Rasmussen
,
date:
04/05/2019,
view:
584,
Q&A:
0
Maize cells are surrounded by cell walls that can be easily visualized using a simple protocol outlined below. A glue impression is an imprint of maize epidermal cells on a glass slide made using a cyanoacrylate-based glue (super glue) that produces ...
Mating and Tetrad Dissection in
Chlamydomonas
Author:
Frej Tulin
,
date:
04/05/2019,
view:
255,
Q&A:
0
The ability to carry out controlled genetic crosses has contributed to making the unicellular green alga
Chlamydomonas reinhardtii
an important model organism for the green plant kingdom (Merchant
et al.
, 2007). This protocol ...
Agrobacterium
-mediated Transformation of
Nicotiana tabacum
cv. Xanthi Leaf Explants
Authors:
Ana Lilia Rosales-Campos
and
Abel Gutiérrez-Ortega
,
date:
01/20/2019,
view:
986,
Q&A:
0
Plant transformation has become an important technology for the large-scale production of a number of goods, ranging from vaccines and pharmaceutical products to biofuel. A plant that is frequently used for this purpose is
Nicotiana tabacum
...
A Simplified Hydroponic Culture of
Arabidopsis
Authors:
Houqing Zeng
,
Chao Xia
,
Cankui Zhang
and
Li-Qing Chen
,
date:
12/20/2018,
view:
1224,
Q&A:
0
Hydroponic culture systems are widely used in research due to their intrinsic properties, such as easily altering nutrient composition, applying chemical treatments like metal, salt and hormones in the growth media, and collecting the root sample. ...
Screening and Genetic Analysis of Ethylene-Response Mutants in Etiolated Rice Seedlings
Authors:
Cui-Cui Yin
,
Biao Ma
,
He Zhao
,
Shou-Yi Chen
and
Jin-Song Zhang
,
date:
09/05/2018,
view:
1226,
Q&A:
0
Ethylene, the simplest gaseous plant phytohormone, is involved in the control of rice growth and development processes, but the mechanism of ethylene regulating these pathways remains unclear in rice. Recent studies have found that ...
Growth of
Chlamydomonas reinhardtii
under Circadian Conditions
Authors:
Volker Wagner
and
Maria Mittag
,
date:
08/20/2018,
view:
1483,
Q&A:
0
The green biflagellate unicellular alga
Chlamydomonas reinhardtii
serves as a model to study fundamental biological processes such as the structure and function of flagella or light-driven processes including photosynthesis, its behavioral ...