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A high-quality database for basic life science protocols
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A high-quality database for basic life science protocols
Plant Science
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Tail Tendon Break Time for the Assessment of Aging and Longitudinal Healthspan in Mice
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Detecting Spaciotemporal Transcript Accumulation in Maize by RNA
In Situ
Hybridization
Authors:
Josh Strable
and
James W. Satterlee
,
date:
02/20/2021,
view:
59,
Q&A:
0
RNA
in situ
hybridization is a method for visualizing spatiotemporal transcript accumulation in cells and tissues. The method provides clear resolution, is highly sensitive and specific, and can uncover gradients of transcript accumulation ...
Optimize Highly Efficient Genetic Transformation Method of Spring Wheat (
Triticum aestivum
L.) Cultivars Using Mature Embryo
Authors:
Dongjin Kim
and
Megan Hager
,
date:
11/20/2020,
view:
404,
Q&A:
0
Genetic engineering is a powerful tool to develop desired traits in crop plants to make the crops suitable for future demand and changing environments. For the genetic engineering of wheat cultivars, the development of efficient transformation and ...
A Low-volume Hydroponic Protocol for Maize and A Sensitive Bud-length Assay for Root-to-Shoot Impacts of The Strigolactone Analog,
rac
-GR24
Authors:
Jiahn-Chou Guan
and
Karen E. Koch
,
date:
08/20/2020,
view:
674,
Q&A:
0
Hydroponic approaches have been a boon for root research by facilitating root-feeding studies as well as secretion analysis. Results have aided our understanding of root-shoot signaling, transport, hormone function and more. However, existing ...
Seed Sterilization and Seedling Growth on Plates in the Model Grass
Brachypodium distachyon
Authors:
Aylin S Haas
and
Michael T. Raissig
,
date:
08/05/2020,
view:
888,
Q&A:
0
Brachypodium distachyon
is a model grass closely related to wheat and barley. New resources and methods are being developed and
Brachypodium
is becoming a popular model for developmental biology, cell wall biology, (phylo) ...
Preparing Immunolocalization Slides of Maize Meiotic Chromosomes for Three-dimensional Microscopy
Authors:
Qian Du
and
Wojciech P. Pawlowski
,
date:
07/20/2020,
view:
668,
Q&A:
0
The protocol provides fully detailed steps for preparing microscopic slides of acrylamide-embedded maize meiotic cells. This method is particularly useful for examining chromatin structure and chromosome arrangement without destroying the ...
Barley Transformation by Particle Bombardment Using Callus
Author:
Sung-Yong Kim
,
date:
06/05/2020,
view:
1026,
Q&A:
0
The delivery of desired construct into plant cells relies mainly on
Agrobacterium
-mediated transformation. However,
Agrobacterium
transformation is still very challenging for barley as only one genotype is amendable to
...
Toluidine Blue O Staining of Paraffin-Sectioned Maize Tissue
Authors:
Josh Strable
,
Jeffrey R. Yen
,
Michael J. Scanlon
and
Anne W. Sylvester
,
date:
05/05/2020,
view:
1422,
Q&A:
0
Histology incorporates microtechnique, the preparation of living material for observation and microscopy to study the anatomy of cells, tissues and organs. Interpreting histological patterns in a specimen may help to correlate form and function at ...
Chiasma Counting in Maize Male Meiocytes
Authors:
Gaganpreet K. Sidhu
and
Wojciech P. Pawlowski
,
date:
05/05/2020,
view:
1194,
Q&A:
0
This protocol combines a classical chromosome spreading technique with 3-dimensional image reconstruction for visualization of bivalent configurations during meiosis to aid chiasma counting.
Immunolabeling of Maize Meiocytes
Authors:
Mateusz Zelkowski
and
Wojciech P. Pawlowski
,
date:
04/05/2020,
view:
1444,
Q&A:
0
This protocol describes a quick method for immunolocalization of meiotic proteins in maize. It includes a fixation step that allows for long-term storage of material and provides good preservation of chromatin structure.
In vitro
Conditions for Dark Growth and Analysis of Maize Seedlings
Authors:
Jeffrey Farrow
,
Marschal A. Bellinger
and
Carolyn G. Rasmussen
,
date:
03/20/2020,
view:
1145,
Q&A:
0
Maize is an important model organism for understanding plant traits essential for proper growth and germination. One type of growth, skotomorphogenesis, occurs in the absence of light. Seedlings grown in the absence of light exhibit dramatic ...
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