Microbiology

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    Protocols in Current Issue
    Bacterial Intracellular Sodium Ion Measurement using CoroNa Green
    Authors:  Yusuke V. Morimoto, Keiichi Namba and Tohru Minamino, date: 01/05/2017, view: 5179, Q&A: 0
    [Abstract] The bacterial flagellar type III export apparatus consists of a cytoplasmic ATPase complex and a transmembrane export gate complex, which are powered by ATP and proton motive force (PMF) across the cytoplasmic membrane, respectively, and transports flagellar component proteins from the cytoplasm to the distal end of the growing flagellar structure ...
    Measurement of Extracellular Ca2+ Influx and Intracellular H+ Efflux in Response to Glycerol and PEG6000 Treatments
    Authors:  Tao Li and Baodong Chen, date: 09/20/2013, view: 6495, Q&A: 0
    [Abstract] The characteristics of Ca2+ and H+ fluxes may reflect the activities of aquaporins, as the up-regulation of aquaporin activities is directly associated with the decrease in cytoplasmic H+ concentration and increase in cytoplasmic Ca2+ concentration. The higher aquaporin activities can protect cells ...
    Detection of Membrane Potential in Mycobacterium tuberculosis
    Authors:  Manbeena Chawla and Amit Singh, date: 06/05/2013, view: 9675, Q&A: 2
    [Abstract] DiOC2 (Novo et al., 2000) exhibits green fluorescence in all bacterial cells, but the fluorescence shifts towards red emission as the dye molecules self associate at the higher cytosolic concentrations caused by larger membrane potentials. Proton ionophores such as CCCP destroy membrane potential by eliminating the proton ...



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