Biochemistry

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    Protocols in Current Issue
    Extraction and Quantification of Polyphosphate in the Budding Yeast Saccharomyces cerevisiae
    Authors:  David Canadell, Samuel Bru, Josep Clotet and Joaquín Ariño, date: 07/20/2016, view: 9863, Q&A: 0
    [Abstract] Inorganic polyphosphate (polyP) is a linear polymer present in both prokaryotic and eukaryotic organisms and made from three to hundreds of orthophosphate residues linked by phosphoanhydride bonds. The biological role of this molecule goes beyond serving as Pi store or energy source to replace ATP. For instance, in yeast polyP levels have been ...
    Extraction and Quantification of Poly P, Poly P Analysis by Urea-PAGE
    Author:  Maria R. Gomez Garcia, date: 05/05/2014, view: 8093, Q&A: 1
    [Abstract] Inorganic polyphosphate (poly P) molecules, linear chains containing hundreds of orthophosphate (Pi) residues linked by high-energy phosphoanhydride bonds are abundant in every cell in nature. These molecules are widely distributed among bacteria, including key pathogens, and eukaryotes, poly P is present in organelles, including nuclei, ...
    Quantification of Total and Soluble Inorganic Phosphate
    Authors:  Vicki Knowles and William Plaxton, date: 09/05/2013, view: 13253, Q&A: 0
    [Abstract] A simple, rapid, and sensitive colorimetric microassay for inorganic phosphate (Pi) relies upon the absorption at 660 nm of a molybdenum blue complex that forms upon reduction of an ammonium molybdate-Pi complex in acid. The method for determination of total Pi uses plant tissues that have been ashed at 500 °C, whereas quantification of soluble Pi ...



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