Cancer Biology

Categories

    Protocols in Current Issue
    Hyaluronan Isolation from Mouse Mammary Gland
    Authors:  Cornelia Tolg, Mary Cowman and Eva A. Turley, date: 06/05/2018, view: 1415, Q&A: 0
    [Abstract] The glycosaminoglycan hyaluronan (HA) is a key component of the extracellular matrix. The molecular weight of HA is heterogeneous and can reach from several million to several hundred daltons. The effect of HA on cell behavior is size dependent; fragmented HA acts as a danger signal, stimulates cell migration and proliferation and is ...
    Liposome Flotation Assays for Phosphoinositide-protein Interaction
    Authors:  Helene Tronchere and Frederic Boal, date: 03/05/2017, view: 8449, Q&A: 0
    [Abstract] Phosphoinositides are rare membrane lipids involved in the control of the major cellular functions and signaling pathways. They are able to recruit specific effector proteins to the cytosolic face of plasma membrane and organelles to coordinate a vast variety of signaling and trafficking processes, as well to maintain specific identity of the ...
    A Technique for the Measurement of in vitro Phospholipid Synthesis via Radioactive Labeling
    Authors:  Lucia Rodriguez-Berdini and Gabriel O. Ferrero, date: 01/20/2016, view: 7745, Q&A: 0
    [Abstract] This is an assay designed to examine the radioactive phosphorous incorporation when the molecule is being synthesized, which means that only de novo synthesized phospholipids can be detected. Thus, with this technique it is possible to detect in vitro phospholipid synthesis under different required experimental conditions respect ...



    We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.