Plant Science

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    Protocols in Current Issue
    Tensile Testing Assay for the Measurement of Tissue Stiffness in Arabidopsis Inflorescence Stem
    Authors:  Kouki Yoshida, Shingo Sakamoto and Nobutaka Mitsuda, date: 08/05/2019, view: 391, Q&A: 0
    [Abstract] Lignocellulosic biomass is a versatile renewable resource for fuels, buildings, crafts, and biomaterials. Strategies of molecularly designing lignocellulose for industrial application has been developed by the discoveries of novel genes after the screenings of various mutants and transformed lines of Arabidopsis whose cell walls could be ...
    Estimation of Stomatal Aperture in Arabidopsis thaliana Using Silicone Rubber Imprints
    Authors:  Telma E. Scarpeci, María I. Zanor and Estela M. Valle, date: 06/20/2017, view: 4970, Q&A: 2
    [Abstract] Estimation of stomatal aperture using low viscosity silicone-base impression material has the advantage of working with the whole leaf. The developmental stage and the environment strongly affect the stomatal aperture. Therefore, it is mandatory to have accurate estimations of the stomatal aperture of intact leaves under different situations. With ...
    [14C] Glucose Cell Wall Incorporation Assay for the Estimation of Cellulose Biosynthesis
    Authors:  Chad Brabham, Jozsef Stork and Seth Debolt, date: 09/20/2015, view: 4200, Q&A: 0
    [Abstract] Cellulose is synthesized by Cellulose Synthase A proteins at the plasma membrane using the substrate UDP glucose. Herein, we provide a detailed method for measuring the incorporation of radiolabeled glucose into the cellulose fraction of the cell wall. In this method Arabidopsis seedlings are treated for 2 h with a cellulose biosynthesis ...



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