Neuroscience

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    Protocols in Current Issue
    Identification of Socially-activated Neurons
    Authors:  Mary L. Phillips and Lucas Pozzo-Miller, date: 09/05/2020, view: 3091, Q&A: 0
    [Abstract] Determining the neuronal circuitry responsible for specific behaviors is a major focus in the field of neurobiology. Activity-dependent immediate early genes (IEGs), transcribed and translated shortly after neurons discharge action potentials, have been used extensively to either identify or gain genetic access to neurons and brain regions ...
    A Novel Protocol to Quantitatively Measure the Endocytic Trafficking of Amyloid Precursor Protein (APP) in Polarized Primary Neurons with Sub-cellular Resolution
    Authors:  Florent Ubelmann, Tatiana Burrinha and Cláudia Guimas Almeida, date: 12/05/2017, view: 5368, Q&A: 0
    [Abstract] Alzheimer’s disease’s established primary trigger is β-amyloid (Aβ) (Mucke and Selkoe, 2012). The amyloid precursor protein (APP) endocytosis is required for Aβ generation at early endosomes (Rajendran and Annaert, 2012). APP retention at endosomes depends on its sorting for degradation in lysosomes (Haass et al., 1992; Morel et al. ...
    Optogenetic Mapping of Synaptic Connections in Mouse Brain Slices to Define the Functional Connectome of Identified Neuronal Populations
    Authors:  Susana Mingote, Nao Chuhma and Stephen Rayport, date: 01/05/2017, view: 8241, Q&A: 0
    [Abstract] Functional connectivity in a neural circuit is determined by the strength, incidence, and neurotransmitter nature of its connections (Chuhma, 2015). Using optogenetics the functional synaptic connections between an identified population of neurons and defined postsynaptic target neurons may be measured systematically in order to determine the ...



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