Biochemistry

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    Protocols in Current Issue
    Fibrin Breakdown Assay
    Authors:  Jiayue Ling, Connor M. Blair and George S. Baillie, date: 04/20/2020, view: 1509, Q&A: 0
    [Abstract] Fibrinolysis is an integral part of the matrix remodeling process that contributes to tissue repair. Fibrin clots are broken down during fibrinolysis in a controlled process. Fibrin degradation products (FDPs) have also been shown to have a role in the regulation of cell growth and are implicated in various vascular diseases. This protocol was ...
    Measuring cAMP Specific Phosphodiesterase Activity: A Two-step Radioassay
    Authors:  Connor M. Blair, Jiayue Ling and George S. Baillie, date: 04/05/2020, view: 848, Q&A: 0
    [Abstract] Cyclic nucleotide degrading phosphodiesterase (PDE) enzymes are crucial to the fine tuning of cAMP signaling responses, playing a pivotal role in regulating the temporal and spatial characteristics of discrete cAMP nanodomains and hence the activity of cAMP-effector proteins. As a consequence of orchestrating cAMP homeostasis, dysfunctional PDE ...
    The Long-lived Protein Degradation Assay: an Efficient Method for Quantitative Determination of the Autophagic Flux of Endogenous Proteins in Adherent Cell Lines
    Authors:  Morten Luhr, Frank Sætre and Nikolai Engedal, date: 05/05/2018, view: 6175, Q&A: 0
    [Abstract] Autophagy is a key player in the maintenance of cellular homeostasis in eukaryotes, and numerous diseases, including cancer and neurodegenerative disorders, are associated with alterations in autophagy. The interest for studying autophagy has grown intensely in the last two decades, and so has the arsenal of methods utilised to study this highly ...
    Ubiquitin Proteasome Activity Measurement in Total Plant Extracts
    Authors:  Suayib Üstün and Frederik Börnke, date: 09/05/2017, view: 5760, Q&A: 0
    [Abstract] The fine-tuned balance of protein level, conformation and location within the cell is vital for the dynamic changes required for a cell to respond to a given stimulus. This requires the regulated turnover of damaged or short-lived proteins through the ubiquitin proteasome system (UPS). Thus, the protease activity of the proteasome is adjusted to ...
    Chase Assay of Protein Stability in Haloferax volcanii
    Authors:  Xian Fu and Julie A. Maupin-Furlow, date: 03/20/2017, view: 4689, Q&A: 0
    [Abstract] Highly regulated and targeted protein degradation plays a fundamental role in almost all cellular processes. Determination of the protein half-life by the chase assay serves as a powerful and popular strategy to compare the protein stability and study proteolysis pathways in cells. Here, we describe a chase assay in Haloferax volcanii, a ...
    Cycloheximide Assays to Measure Protein Degradation in vivo in Plants
    Authors:  Jonathan Gilkerson, Raymond Tam, Aimee Zhang, Kate Dreher and Judy Callis, date: 09/05/2016, view: 12799, Q&A: 0
    [Abstract] The half-life of a protein is a characteristic property, and can be modulated by post-translational modifications, changes in subcellular localization, and/or interaction with other proteins or ligands. As one determinant of its steady-state level, a protein’s degradation represents an important distinguishing attribute relevant to its biological ...



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