Cancer Biology

Categories

    Protocols in Current Issue
    The Chick Embryo Chorioallantoic Membrane as an in vivo Model to Study Metastasis
    Authors:  Piero Crespo and Berta Casar, date: 10/20/2016, view: 11907, Q&A: 0
    [Abstract] Metastasis is a complex process that includes several steps: neoplastic progression, angiogenesis, cell migration and invasion, intravasation into nearby blood vessels, survival in the circulatory system, extravasation followed by homing into distant tissues, the formation of micrometastases, and finally the growth into macroscopic secondary ...
    In vitro Tumor Cell Migration Assay Using ThinCertsTM (Transwells)
    Author:  Marc A. Schneider, date: 06/05/2016, view: 11317, Q&A: 0
    [Abstract] The high migration rate of tumor cells often results in poor prognosis for the survival of the patients. Here, we describe a protocol to measure the migration of cells using a quantitative assay. The relative tumor cell migration was measured using ThinCertsTM cell culture inserts and a lactate dehydrogenase (LDH) assay to quantify the ...
    Colon Cancer-associated Fibroblast Establishment and Culture Growth
    [Abstract] Cancer-associated fibroblasts (CAFs) are one of the major players in tumor-stroma crosstalk. Findings in experimental studies suggest important roles for CAFs in regulation of tumor growth, metastasis and drug response (Hanahan and Coussens, 2012). Furthermore, their clinical relevance is supported by new findings from tumor analyses, ...
    In vitro Flow Adhesion Assay for Analyzing Shear-resistant Adhesion of Metastatic Cancer Cells to Endothelial Cells
    Authors:  Shin-Ae Kang, Sandra Bajana and Takemi Tanaka, date: 02/20/2016, view: 8345, Q&A: 1
    [Abstract] Hematogenous metastasis is a primary cause of mortality from metastatic cancer. The shear-resistant adhesion of circulating tumor cells to the vascular endothelial cell surface under blood flow is an essential step in cell extravasation and further tissue invasion. This is similar to a process exploited by leukocytes for adhesion to inflamed blood ...
    PhagoKinetic Track Assay: Imaging and Analysis of Single Cell Migration
    [Abstract] Cell migration is a highly complex and dynamic biological process, essential in several physiological phenomena and pathologies including cancer dissemination and metastasis formation. Thus understanding single cell migration is highly relevant and requires a suitable image-based assay. Depending on the speed of the moving cells, one may require ...
    Hanging Drop Aggregation Assay of Breast Cancer Cells
    Author:  Yong Teng, date: 02/05/2015, view: 13085, Q&A: 0
    [Abstract] Hanging drop assay can be used to investigate cell-cell cohesion and cell-substratum adhesion through generation of 3D spheroids under physiological conditions. It also can be used to elucidate the role of cell-cell or cell-ECM interactions in specifying spatial relationships between two (or more) different cell populations. This simple method ...
    Three Dimensional Spheroid Co-culture Invasion Assay
    Authors:  Eldo T Verghese and Thomas A Hughes, date: 01/05/2015, view: 9070, Q&A: 0
    [Abstract] The assay was developed to investigate the impact of stromal cells of different types (in our case breast cancer associated fibroblasts stably manipulated to modify expression of genes of interest) on the invasive capacity of epithelial cancer cells (in our case breast cancer cell lines) (Verghese et al., 2013). Typical two dimensional ...
    Invadopodia Detection and Gelatin Degradation Assay
    Author:  Begoña Díaz, date: 12/20/2013, view: 15677, Q&A: 0
    [Abstract] This protocol is designed to quantify invadopodia formation and activity. Invadopodia are protrusive structures elaborated by cancer cells that mediate cell attachment and remodeling of the extracellular matrix. These structures contribute to the ability of cancer cells to invade and metastasize. In this protocol, both the presence of invadopodia ...
    Three-dimensional Invasion Assay
    Authors:  Wen-Hao Yang and Muh-Hwa Yang, date: 09/05/2013, view: 7774, Q&A: 0
    [Abstract] The invasive ability of cancer cells is a crucial function for cancer metastasis and the surrounding microenvironment of cancer cells in living tissues is three-dimension (3D). Therefore, to establish an in vitro invasion assay in a 3D system to predict cancer invasive ability is valuable in the research for cancer metastasis. Here, we ...
    Scratch Wound Healing Assay
    Author:  Yanling Chen, date: 03/05/2012, view: 98730, Q&A: 6
    [Abstract] The scratch wound healing assay has been widely adapted and modified to study the effects of a variety of experimental conditions, for instance, gene knockdown or chemical exposure, on mammalian cell migration and proliferation. In a typical scratch wound healing assay, a “wound gap” in a cell monolayer is created by scratching, and the “healing” ...



    We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.