Biochemistry

Categories

    Protocols in Current Issue
    Fluorescence-based Heme Quantitation in Toxoplasma Gondii
    Authors:  Amy Bergmann and Zhicheng Dou, date: 06/20/2021, view: 1045, Q&A: 0
    [Abstract]

    Toxoplasma gondii is a highly prevalent protozoan pathogen throughout the world. As a eukaryotic intracellular pathogen, Toxoplasma ingests nutrients from host cells to support its intracellular growth. The parasites also encode full or partial metabolic pathways for the biosynthesis of certain nutrients, such as heme. Heme is an essential

    ...
    Pyridine Hemochromagen Assay for Determining the Concentration of Heme in Purified Protein Solutions
    Authors:  Ian Barr and Feng Guo, date: 09/20/2015, view: 27616, Q&A: 2
    [Abstract] Heme is a common cofactor in proteins, found in hemoglobin, myoglobin, cytochrome P450, DGCR8, and nitric oxide synthase, among others. This protocol describes a method for quantifying heme that works best in purified protein samples. This protocol might be used to, for example, determine whether a given heme-binding protein is fully occupied by ...



    We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.