Plant Science

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    Protocols in Current Issue
    Low-cost and High-throughput RNA-seq Library Preparation for Illumina Sequencing from Plant Tissue
    Authors:  Marta Bjornson, Kaisa Kajala, Cyril Zipfel and Pingtao Ding, date: 10/20/2020, view: 3227, Q&A: 0
    [Abstract] Transcriptome analysis can provide clues to biological processes affected in different genetic backgrounds or/and under various conditions. The price of RNA sequencing (RNA-seq) has decreased enough so that medium- to large-scale transcriptome analyses in a range of conditions are feasible. However, the price and variety of options for library ...
    Dot Blot Analysis of N6-methyladenosine RNA Modification Levels
    Authors:  Lisha Shen, Zhe Liang and Hao Yu, date: 01/05/2017, view: 21340, Q&A: 3
    [Abstract] N6-methyladenosine (m6A) is the most prevalent internal modification of eukaryotic messenger RNA (mRNA). The total amount of m6A can be detected by several methods, such as dot blot analysis using specific m6A antibodies and quantitative liquid chromatography-tandem mass spectrometry (LC-MS/MS) (Fu et al ...
    Separation of Microspores from Anthers of Lilium longiflorum (Lily) and Subsequent RNA Extraction
    Authors:  Ming-Che Liu, Yun-Chu Chen and Co-Shine Wang, date: 02/20/2015, view: 8551, Q&A: 0
    [Abstract] This protocol has been designed in order to facilitate the isolation and extraction of total RNA from microspores collected from lily anther sacs. This protocol allows the extraction of high amounts of high quality RNA, as observed in agarose gels.



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