Lisha Shen Temasek Life Sciences Laboratory and Department of Biological Sciences, National University Singapore,

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Hao Yu Temasek Life Sciences Laboratory and Department of Biological Sciences, National University of Singapore, Singapore
1 protocol

Zhe Liang Temasek Life Sciences Laboratory and Department of Biological Sciences, National University of Singapore, Singapore
1 protocol

Lisha Shen

Temasek Life Sciences Laboratory and Department of Biological Sciences, National University of Singapore, Singapore

Research focus

Plant science

1 Author merit
Reviewer merit
Editor merit

1 Protocol published

Dot Blot Analysis of N⁶-methyladenosine RNA Modification Levels

Authors: Lisha Shen, Zhe Liang and Hao Yu, date: 01/05/2017, view: 24197, Q&A: 3

N⁶-methyladenosine (m⁶A) is the most prevalent internal modification of eukaryotic messenger RNA (mRNA). The total amount of m⁶A can be detected by several methods, such as dot blot analysis using specific m⁶ ...

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Alex Wardale

Nord University

Hello. I cannot get this protocol to work, I was wondering if you have a reference troubleshooting guide? I use the same primary and secondary antibodies, purified and serial diluted mRNA, and same buffers, the only difference being I use BSA instead of non-fat milk.

4/27/2021 11:58:48 PM Reply

Hao Yu

Temasek Life Sciences Laboratory and Department of Biological Sciences, National University of Singapore, Singapore

There are many factors determining whether you can get good signals of dot blot, such as mRNA qualities, m6A abundance in the samples, crosslinking efficiency, etc. We suggest you to include some positive controls and try again.

2021-05-09 04:14:45

Alex Wardale

Nord University

This issue has been resolved.

2021-04-29 01:02:09