Plant Science

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    Protocols in Current Issue
    Radioactive Tracer Feeding Experiments and Product Analysis to Determine the Biosynthetic Capability of Comfrey (Symphytum officinale) Leaves for Pyrrolizidine Alkaloids
    Authors:  Thomas Stegemann, Lars H. Kruse and Dietrich Ober, date: 02/05/2018, view: 1846, Q&A: 0
    [Abstract] This protocol delivers a method to determine the biosynthetic capability of comfrey leaves for pyrrolizidine alkaloids independently from other organs like roots or flowers.

    The protocol applies and combines radioactive tracer experiments with standard and modern techniques like thin layer chromatography (TLC), solid-phase extraction ...
    1-MCP (1-methylcyclopropene) Treatment Protocol for Fruit or Vegetables
    Authors:  Gamrasni Dan, Goldway Martin, Stern Yosi, Breitel Dario and Aharoni Asaph, date: 05/20/2017, view: 6240, Q&A: 4
    [Abstract] 1-MCP (1-methylcyclopropene) is a simple synthetic hydrocarbon molecule that interacts with the ethylene receptor and inhibits the response of fruit or plant to ethylene. 1-MCP has opened new opportunities in handling harvested crops and serves as a powerful tool to learn about plant response to ethylene (Watkins and Miller, 2006). 1-MCP is ...
    Extraction and Analysis of Carotenoids from Escherichia coli in Color Complementation Assays
    Authors:  Andreas Blatt and Martin Lohr, date: 03/20/2017, view: 5748, Q&A: 0
    [Abstract] A common method to investigate the function of genes putatively involved in carotenoid biosynthesis is the so called color complementation assay in Escherichia coli (see, e.g., Cunningham and Gantt, 2007). In this assay, the gene under investigation is expressed in E. coli strains genetically engineered to synthesize ...
    Acetyl Bromide Soluble Lignin (ABSL) Assay for Total Lignin Quantification from Plant Biomass
    Authors:  William J. Barnes and Charles T. Anderson, date: 03/05/2017, view: 7114, Q&A: 0
    [Abstract] Lignin is the second most abundant biopolymer on Earth, providing plants with mechanical support in secondary cell walls and defense against abiotic and biotic stresses. However, lignin also acts as a barrier to biomass saccharification for biofuel generation (Carroll and Somerville, 2009; Zhao and Dixon, 2011; Wang et al., 2013). For ...
    Rice Root Organic Acid Efflux Measurement by Using Ion Chromatography
    Authors:  Chun-quan Zhu, Xiao-fang Zhu and Ren-fang Shen, date: 02/20/2017, view: 4135, Q&A: 0
    [Abstract] Organic acids secreted from plant roots play important roles in various biological processes including nutrient acquisition, metal detoxification, and pathogen attraction. The secretion of organic acids may be affected by various conditions such as plant growth stage, nutrient deficiency, and abiotic stress. For example, when white lupin (Lupinus ...
    Hydrogen Peroxide Measurement in Arabidopsis Root Tissue Using Amplex Red
    Authors:  Tzvetina Brumbarova, Cham Thi Tuyet Le and Petra Bauer, date: 11/05/2016, view: 7463, Q&A: 0
    [Abstract] This protocol describes the measurement of hydrogen peroxide (H2O2) content in Arabidopsis root tissue by using the Amplex® Red Hydrogen Peroxide/Peroxidase Assay Kit. When root tissue is disrupted and resuspended in phosphate buffer, H2O2 is released from the cells. The obtained root ...
    Isolation of Flavonoids from Piper delineatum Leaves by Chromatographic Techniques
    Authors:  Isabel L. Bazzocchi, Juan C. Ticona, Ignacio A. Jiménez and Ninoska Flores, date: 07/20/2016, view: 9378, Q&A: 0
    [Abstract] The genus Piper (Piperaceae) is widely distributed in the tropical and subtropical regions of the world, and species belonging to this genus are included in the Ayurvedic system of medicine and in folklore medicine of Latin America. Phytochemical investigations of Piper species have led to the isolation of several classes of ...
    Detection and Quantification of Heme and Chlorophyll Precursors Using a High Performance Liquid Chromatography (HPLC) System Equipped with Two Fluorescence Detectors
    Authors:  Jan Pilný, Jana Kopečná, Judith Noda and Roman Sobotka, date: 02/05/2015, view: 7292, Q&A: 0
    [Abstract] Intermediates of tetrapyrrole biosynthetic pathway are low-abundant compounds, and their quantification is usually difficult, time consuming and requires large amounts of input material. Here, we describe a method allowing fast and accurate quantification of almost all intermediates of the heme and chlorophyll biosynthesis, including mono-vinyl ...
    Application of Silicone Tubing for Robust, Simple, High-throughput, and Time-resolved Analysis of Plant Volatiles in Field Experiments
    Authors:  Mario Kallenbach, Daniel Veit, Elisabeth J. Eilers and Meredith C. Schuman, date: 02/05/2015, view: 7800, Q&A: 0
    [Abstract] Plant volatiles (PVs) mediate manifold interactions between plants and their biotic and abiotic environments (Dicke and Baldwin, 2010; Holopainen and Gershenzon, 2010). An understanding of the physiological and ecological functions of PVs must therefore be based on measurements of PV emissions under natural conditions. Yet sampling PVs in natural ...
    Collection of Root Exudate from Duckweed
    Authors:  Yufang Lu, Li Sun and Weiming Shi, date: 01/05/2015, view: 8800, Q&A: 0
    [Abstract] Root exudates play an important role in rhizosphere interactions between plants and microorganisms. However, collection of chemicals from plant root system is difficult due to their low concentrations and high level of contaminants in growth media. The continuous collection method has been described in several terrestrial plants over the past 30 ...



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