Cell Biology

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    Protocols in Current Issue
    Quantitative Live-cell Reporter Assay for Noncanonical Wnt Activity
    Authors:  Edith P. Karuna, Michael W. Susman and Hsin-Yi Henry Ho, date: 03/20/2018, view: 2935, Q&A: 0
    [Abstract] Noncanonical Wnt signaling functions independently of the β-catenin pathway to control diverse developmental processes, and dysfunction of the pathway contributes to a number of human pathological conditions, including birth defects and metastatic cancer. Progress in the field, however, has been hampered by the scarcity of functional assays for ...
    1-MCP (1-methylcyclopropene) Treatment Protocol for Fruit or Vegetables
    Authors:  Gamrasni Dan, Goldway Martin, Stern Yosi, Breitel Dario and Aharoni Asaph, date: 05/20/2017, view: 6296, Q&A: 4
    [Abstract] 1-MCP (1-methylcyclopropene) is a simple synthetic hydrocarbon molecule that interacts with the ethylene receptor and inhibits the response of fruit or plant to ethylene. 1-MCP has opened new opportunities in handling harvested crops and serves as a powerful tool to learn about plant response to ethylene (Watkins and Miller, 2006). 1-MCP is ...
    Detection of Wnt5 in Media Conditioned by Mouse Embryonic Fibroblast
    Authors:  Juan Flores and Nan Gao, date: 10/20/2016, view: 4267, Q&A: 0
    [Abstract] This protocol describes the procedure of visualizing secreted Wnt5 protein in serum free media via western blotting. This procedure can also be used to visualize other secreted proteins larger than 10,000 daltons. The work presented in this paper visualizes Wnt5 secreted by mouse embryonic fibroblast (MEF), but can be adapted to other cell lines ...
    TGFβ Release Co-culture Assay
    Author:  Emanuel Rognoni, date: 12/05/2014, view: 6500, Q&A: 0
    [Abstract] TGFβ is a potent cytokine modulating various processes including proliferation, differentiation, ECM synthesis and apoptosis (Siegel and Massague, 2003). Thus in many tissues availability of TGFβ is tightly regulated. TGFβ is secreted as an inactive complex where it is encapsulated by the latency associated protein (LAP), a ligand trap protein, ...
    Separation of the Inner and Outer Mitochondrial Membrane in HeLa Cells
    Authors:  Naotaka Nishimura and Masato Yano, date: 11/20/2014, view: 15954, Q&A: 1
    [Abstract] Mitochondria are organelles that have important functions in oxidative phosphorylation, fatty acid oxidation and apoptosis signaling. They have two distinct membranes, outer membrane (OM) and inner membrane (IM). IM contains respiratory chain complexes that produce ATP. IM is rich in cardiolipin, a specific phospholipid reportedly having a ...
    Wnt Reporter Activity Assay
    Author:  Chen Zhao, date: 07/20/2014, view: 19939, Q&A: 1
    [Abstract] This protocol is for testing responses of a candidate cell line/cell lines to Wnt ligands or Wnt pathway agonists stimulation. This protocol can also be adapted to screen small molecule libraries or biologics that contain activities to either increase or decrease Wnt pathway responses. Canonical Wnt signaling activity transcriptionally induces Wnt ...
    Hedgehog (Hh) Reporter Activity Assay
    Author:  Chen Zhao, date: 07/20/2014, view: 9587, Q&A: 0
    [Abstract] This protocol is for testing responses of a candidate cell line/cell lines to Hh ligands or Hh pathway agonists stimulation. This protocol can also be adapted to screen small molecule libraries or biologics that contain activities to either increase or decrease Hh pathway responses. Canonical Hh signaling activity transcriptionally induces Hh ...



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