Cell Biology

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    Protocols in Current Issue
    MHC Class II Tetramer Labeling of Human Primary CD4+ T Cells from HIV Infected Patients
    [Abstract] Major Histocompatibility Complex (MHC) tetramers have been used for two decades to detect, isolate and characterize T cells specific for various pathogens and tumor antigens. In the context of Human Immunodeficiency Virus (HIV) infection, antigen-specific CD8+ T cells have been extensively studied ex vivo, as they can be ...
    Histochemical Detection of Zn in Plant Tissues
    Authors:  Ilya Seregin, Anna Kozhevnikova and Henk Schat, date: 05/20/2015, view: 6016, Q&A: 0
    [Abstract] Accumulation of metals in plant tissues, and occasionally, different cells of the same tissue, may be highly non-uniform (Seregin and Kozhevnikova, 2008). Easy-to-use histochemical methods may greatly help to investigate the distribution and accumulation of metals within and among plant tissues, and also provide information on their subcellular ...
    Calcium Mobilization Assay to Measure the Activity of Gq-coupled Receptors
    Author:  Hamiyet Unal, date: 06/20/2013, view: 14473, Q&A: 0
    [Abstract] Calcium mobilization assay is a cell-based second messenger assay to measure the calcium flux associated with Gq-protein coupled receptor activation or inhibition. The method utilizes a calcium sensitive fluorescent dye that is taken up into the cytoplasm of most cells. In some cell lines in which organic-anion transporters are particularly active ...
    Detection of Membrane Potential in Mycobacterium tuberculosis
    Authors:  Manbeena Chawla and Amit Singh, date: 06/05/2013, view: 9384, Q&A: 2
    [Abstract] DiOC2 (Novo et al., 2000) exhibits green fluorescence in all bacterial cells, but the fluorescence shifts towards red emission as the dye molecules self associate at the higher cytosolic concentrations caused by larger membrane potentials. Proton ionophores such as CCCP destroy membrane potential by eliminating the proton ...
    Culture and Detection of Mycobacterium tuberculosis (MTB) and Mycobacterium bovis (BCG)
    Author:  Ran Chen, date: 01/20/2012, view: 27299, Q&A: 1
    [Abstract] Mycobacterium tuberculosis (MTB) is the bacterial pathogen responsible for tuberculosis, a human pulmonary infectious disease. Mycobacterium bovis (BCG) is the causative agent of tuberculosis in cattle, and is often used as the vaccine stain in humans. Specific recipes and methods for culture of MTB and BCG are described in this ...



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