Alessandro Didonna East Carolina University
80 protocols

Alka Mehra Institute of Genomics and Integrative Biology
46 protocols

Chhuttan Meena National Chemical laboratory NCL Pune
6 protocols

David Cisneros Umeå University
33 protocols

Reviewer
Ran Chen
  • CEO, Zhejiang JFK Biological Technology Co. Ltd.
Research focus
  • Biochemistry
  • 7 Author merit
7 Protocols published
Isolation and Culture of Mouse Bone Marrow-derived Macrophages (BMM’phi’)
Author:  Ran Chen, date: 02/05/2012, view: 66661, Q&A: 15
Bone marrow derived macrophages are a type of white blood cell that can be isolated from mammalian bone marrow. In this protocol, a method is described in which bone marrow cells are isolated from mouse leg bones (femur and tibia), and then ...
MTT Assay of Cell Numbers after Drug/Toxin Treatment
Author:  Ran Chen, date: 04/05/2011, view: 25028, Q&A: 1
MTT assay is a colorimetric method for measuring the activity of enzymes in living cells that reduce MTT to formazan dyes, giving a purple color. It is commonly used to determine cytotoxicity of potential medicinal agents and toxic materials, since ...
6 Protocols reviewed
Optimised Method for the Production and Titration of Lentiviral Vectors Pseudotyped with the SARS-CoV-2 Spike

The use of recombinant lentivirus pseudotyped with the coronavirus Spike protein of SARS-CoV-2 would circumvent the requirement of biosafety-level 3 (BSL-3) containment facilities for the handling of SARS-CoV-2 viruses. Herein, we describe a fast

...
More >
Optimised Method for the Production and Titration of Lentiviral Vectors Pseudotyped with the SARS-CoV-2 Spike

The use of recombinant lentivirus pseudotyped with the coronavirus Spike protein of SARS-CoV-2 would circumvent the requirement of biosafety-level 3 (BSL-3) containment facilities for the handling of SARS-CoV-2 viruses. Herein, we describe a fast

...
More >
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