Cell Biology

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    Protocols in Current Issue
    Human Endothelial Cell Spheroid-based Sprouting Angiogenesis Assay in Collagen
    Authors:  Fabian Tetzlaff and Andreas Fischer, date: 09/05/2018, view: 2595, Q&A: 0
    [Abstract] Angiogenesis, the formation of new blood vessels from pre-existing ones plays an important role during organ development, regeneration and tumor progression. The spheroid-based sprouting assay is a well-established and robust method to study the influence of genetic alterations or pharmacological compounds on capillary-like tube formation of ...
    Fluorescent Labeling of Rat-tail Collagen for 3D Fluorescence Imaging
    Author:  Andrew D. D Doyle, date: 07/05/2018, view: 2272, Q&A: 0
    [Abstract] Rat tail collagen solutions have been used as polymerizable in vitro three-dimensional (3D) extracellular matrix (ECM) gels for single and collective cell migration assays as well as spheroid formation. These 3D hydrogels are a relatively inexpensive, simple to use model system that can mimic the in vivo physical characteristics ...
    Explant Methodology for Analyzing Neuroblast Migration
    Authors:  Kirsty J. Dixon, Alisa Turbic, Ann M. Turnley and Daniel J. Liebl, date: 05/05/2017, view: 4399, Q&A: 0
    [Abstract] The subventricular zone (SVZ) in the mammalian forebrain contains stem/progenitor cells that migrate through the rostral migratory stream (RMS) to the olfactory bulb throughout adulthood. SVZ-derived explant cultures provide a convenient method to assess factors regulating the intermediary stage of neural stem/progenitor cell migration. Here, we ...
    The Chick Embryo Chorioallantoic Membrane as an in vivo Model to Study Metastasis
    Authors:  Piero Crespo and Berta Casar, date: 10/20/2016, view: 10196, Q&A: 0
    [Abstract] Metastasis is a complex process that includes several steps: neoplastic progression, angiogenesis, cell migration and invasion, intravasation into nearby blood vessels, survival in the circulatory system, extravasation followed by homing into distant tissues, the formation of micrometastases, and finally the growth into macroscopic secondary ...
    In vivo Analysis of Neutrophil Infiltration during LPS-induced Peritonitis
    Authors:  Lucia de Almeida, Andrea Dorfleutner and Christian Stehlik, date: 10/05/2016, view: 5324, Q&A: 0
    [Abstract] Bacterial lipopolysaccharide (LPS) is present in the outer membrane of Gram-negative bacteria and functions as pathogen-associated molecular pattern (PAMP) (Whitfield and Trent, 2014). LPS therefore is a potent activator of inflammatory responses leading to cytokine release and neutrophils recruitment. The lipid A moiety of LPS activates the ...
    Hanging Drop Aggregation Assay of Breast Cancer Cells
    Author:  Yong Teng, date: 02/05/2015, view: 11744, Q&A: 0
    [Abstract] Hanging drop assay can be used to investigate cell-cell cohesion and cell-substratum adhesion through generation of 3D spheroids under physiological conditions. It also can be used to elucidate the role of cell-cell or cell-ECM interactions in specifying spatial relationships between two (or more) different cell populations. This simple method ...
    In vitro Migration Assays for Neural Stem Cells, Intermediate Neurogenic Progenitors and Immature Neurons
    Authors:  Julia Ladewig, Philipp Koch and Oliver Brüstle, date: 01/05/2015, view: 10488, Q&A: 0
    [Abstract] In the vertebrate central nervous system (CNS), different neural precursor populations such as neural stem cells (NSCs), intermediate neurogenic progenitors (INPs) and immature neurons have to migrate from their places of birth to their location of function. Coordinated migration is mediated by direct cell-cell interactions and by extracellular ...
    Isolation of Cells from Human Intestinal Tissue
    Authors:  Heli Uronen-Hansson, Emma Persson, Petra Nilsson and William Agace, date: 04/05/2014, view: 11180, Q&A: 0
    [Abstract] The intestinal lamina propria contains a dense network of T cells, dendritic cells (DCs) and macrophages, which play an important role in local innate and adaptive immune responses. We have recently identified distinct subsets of DCs (Persson et al., 2013) and macrophages (Bain et al., 2013) in the human intestine. In addition, ...
    Extravillous Trophoblast Migration and Invasion Assay
    [Abstract] Extravillous trophoblast (EVT) migration and invasion through the decidualized endometrium is essential to successful placentation. SGHPL-4 cells, an EVT cell line derived from first trimester placenta, is a widely used model of cytotrophoblast differentiation into an invasive phenotype. Here we describe a quantitative cell migration assay that ...
    Scratch Wound Healing Assay
    Author:  Yanling Chen, date: 03/05/2012, view: 90975, Q&A: 6
    [Abstract] The scratch wound healing assay has been widely adapted and modified to study the effects of a variety of experimental conditions, for instance, gene knockdown or chemical exposure, on mammalian cell migration and proliferation. In a typical scratch wound healing assay, a “wound gap” in a cell monolayer is created by scratching, and the “healing” ...



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