Plant Science

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    Protocols in Current Issue
    Analysis of Xyloglucan Composition in Arabidopsis Leaves
    Authors:  Javier Sampedro, Cristina Gianzo, Esteban Guitián, Gloria Revilla and Ignacio Zarra, date: 10/05/2017, view: 3976, Q&A: 0
    [Abstract] Xyloglucan is one of the main components of the primary cell wall in most species of plants. This protocol describes a method to analyze the composition of the enzyme-accessible and enzyme-inaccessible fractions of xyloglucan in the model species Arabidopsis thaliana. It is based on digestion with an endoglucanase that attacks ...
    Whole-seed Immunolabeling of Arabidopsis Mucilage Polysaccharides
    Author:  Cătălin Voiniciuc, date: 06/05/2017, view: 4843, Q&A: 0
    [Abstract] In addition to synthesizing and secreting copious amounts of pectic polymers (Young et al., 2008), Arabidopsis thaliana seed coat epidermal cells produce small amounts of cellulose and hemicelluloses typical of secondary cell walls (Voiniciuc et al., 2015c). These components are intricately linked and are released as a ...
    Rapid Determination of Cellulose, Neutral Sugars, and Uronic Acids from Plant Cell Walls by One-step Two-step Hydrolysis and HPAEC-PAD
    Authors:  Trevor Yeats, Tamara Vellosillo, Nadav Sorek, Ana B. Ibáñez and Stefan Bauer, date: 10/20/2016, view: 12157, Q&A: 1
    [Abstract] The plant cell wall is primarily composed of the polysaccharides cellulose, hemicellulose and pectin. The structural and compositional complexity of these components are important for determining cell wall function during plant growth. Moreover, cell wall structure defines a number of functional properties of plant-derived biomass, such as ...
    Analysis of Monosaccharides in Total Mucilage Extractable from Arabidopsis Seeds
    Authors:  Cătălin Voiniciuc and Markus Günl, date: 05/05/2016, view: 7264, Q&A: 0
    [Abstract] The Arabidopsis thaliana seed coat epidermis produces copious amounts of mucilage polysaccharides (Haughn and Western, 2012). Characterization of mucilage mutants has identified novel genes required for cell wall biosynthesis and modification (North et al., 2014). The biochemical analysis of seed mucilage is essential to evaluate ...
    Quantification of the Mucilage Detachment from Arabidopsis Seeds
    Author:  Cătălin Voiniciuc, date: 05/05/2016, view: 5673, Q&A: 0
    [Abstract] The Arabidopsis thaliana seed coat produces large amounts of cell wall polysaccharides, which swell out of the epidermal cells upon hydration of the mature dry seeds. While most mucilage polymers immediately diffuse in the surrounding solution, the remaining fraction tightly adheres to the seed, forming a dense gel-like capsule (Macquet ...
    Assay of Arabinofuranosidase Activity in Maize Roots
    Authors:  Liudmila V. Kozlova, Polina V. Mikshina and Tatyana A. Gorshkova, date: 03/20/2016, view: 6662, Q&A: 0
    [Abstract] Root is a perfect model for studying the mechanisms of plant cell growth. Along the root length, several zones where cells are at different stages of development can be visualized (Figure 1). The dissection of the root on these zones allows the investigation of biochemical and genetic aspects of different growth steps. Maize primary root is much ...
    Immunogold Labeling Analysis of Cell Wall Polysaccharides with Special Reference to (1;3,1;4)-β-D-glucan in Rice Cell Walls
    [Abstract] Various types of cell wall compositions have evolved to fulfill a wide range of biological roles during the diversification of land plants. (1;3,1;4)-β-D-glucan (MLG) is a defining feature of the cell walls in the order Poales (Yokoyama and Nishitani, 2004), which has multiple functions associated with metabolic, growth, and defense systems. MLG ...
    Detection of Hydroxyproline O-galactoside by LC/MS
    Authors:  Mari Ogawa-Ohnishi and Yoshikatsu Matsubayashi, date: 01/20/2016, view: 4994, Q&A: 0
    [Abstract] Hydroxyproline (Hyp) O-galactosylation is a plant-specific post-translational modification found in extracellular glycoproteins such as arabinogalactan proteins (AGPs). Hyp O-galactosylation is mediated by Hyp O-galactosyltransferase (HPGT) that catalyzes the transfer of a D-galactopyranosyl residue to the hydroxyl group ...
    Analysis of Starch Synthase Activities in Wheat Grains using Native-PAGE
    Author:  Zhongyi Li, date: 01/20/2016, view: 8284, Q&A: 0
    [Abstract] Starch synthases are one class of key enzymes involving in the synthesis of cereal starch, which transfer glucose from ADP-glucose to the non-reducing end of pre-existing α-(1-4)-liked glucosyl chains of amylopectin. This protocol is highly reproducible for assaying activities for starch synthase I and IIIa in wheat and barley endosperm at ...
    Saccharification Protocol for Small-scale Lignocellulosic Biomass Samples to Test Processing of Cellulose into Glucose
    Authors:  Rebecca Van Acker, Ruben Vanholme, Kathleen Piens and Wout Boerjan, date: 01/05/2016, view: 8741, Q&A: 0
    [Abstract] Second generation biofuels are derived from inedible lignocellulosic biomass of food and non-food crops. Lignocellulosic biomass is mainly composed of cell walls that contain a large proportion of cellulosic and hemicellulosic polysaccharides. An interesting route to generate biofuels and bio-based materials is via enzymatic hydrolysis of cell ...



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