Plant Science

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    Protocols in Current Issue
    Rice Ragged Stunt Virus Propagation and Infection on Rice Plants
    Authors:  Chao Zhang, Chaonan Shi, Dong Chen and Jianguo Wu, date: 10/20/2018, view: 1559, Q&A: 0
    [Abstract] Virus inoculation is a basic experimental procedure to evaluate the resistance of a rice variety or a transgenic material upon virus infection. We recently demonstrated that Rice Ragged Stunt Virus (RRSV), an oryzavirus that is transmitted by brown planthopper (BPH), can suppress jasmonic acid-mediated antiviral defense through ...
    Fusarium graminearum Inoculation on Wheat Head
    Authors:  Chanjing Feng, Huiquan Liu and Zhe Tang, date: 08/05/2018, view: 2686, Q&A: 0
    [Abstract] Fusarium graminearum, the major causal agent of Fusarium head blight (FHB), causes serious wheat yield losses and a threat to human and animal health. The main efforts to combat the disease are the research of pathogenesis mechanisms and breeding for disease resistance plants. The efficiency of these actions could be evaluated by ...
    Protocol for RYMV Inoculation and Resistance Evaluation in Rice Seedlings
    Authors:  Agnès Pinel-Galzi, Eugénie Hébrard, Oumar Traoré, Drissa Silué and Laurence Albar, date: 06/05/2018, view: 2412, Q&A: 0
    [Abstract] Rice yellow mottle virus (RYMV), a mechanically transmitted virus that causes serious damage to cultivated rice plants, is endemic to Africa. Varietal selection for resistance is considered to be the most effective and sustainable management strategy. Standardized resistance evaluation procedures are required for the identification and ...
    In-vitro and in-planta Botrytis cinerea Inoculation Assays for Tomato
    Authors:  Jiajie Lian, Hongyu Han, Jiuhai Zhao and Chuanyou Li, date: 04/20/2018, view: 4696, Q&A: 0
    [Abstract] Botrytis cinerea (B. cinerea) attacks many crops of economic importance, represents one of the most extensively studied necrotrophic pathogens. Inoculation of B. cinerea and phenotypic analysis of plant resistance are key procedures to investigate the mechanism of plant immunity. Here we describe a protocol for B. ...
    Large Scale Field Inoculation and Scoring of Maize Southern Leaf Blight and Other Maize Foliar Fungal Diseases
    Authors:  Shannon M. Sermons and Peter J. Balint-Kurti, date: 03/05/2018, view: 3177, Q&A: 0
    [Abstract] Field-grown maize is inoculated with Cochliobolus heterostrophus, causal agent of southern leaf blight disease, by dropping sorghum grains infested with the fungus into the whorl of each maize plant at an early stage of growth. The initial lesions produce secondary inoculum that is dispersed by wind and rain, causing multiple cycles of ...
    Quantification of Plant Cell Death by Electrolyte Leakage Assay
    Authors:  Noriyuki Hatsugai and Fumiaki Katagiri, date: 03/05/2018, view: 7770, Q&A: 1
    [Abstract] We describe a protocol to measure the electrolyte leakage from plant tissues, resulting from loss of cell membrane integrity, which is a common definition of cell death. This simple protocol is designed to measure the electrolyte leakage from a tissue sample over a time course, so that the extent of cell death in the tissue can be monitored ...
    Xanthomonas oryzae pv. oryzae Inoculation and Growth Rate on Rice by Leaf Clipping Method
    Authors:  Yinggen Ke, Shugang Hui and Meng Yuan, date: 10/05/2017, view: 8121, Q&A: 0
    [Abstract] Bacterial blight caused by Xanthomonas oryzae pv. oryzae (Xoo) is one of the most serious bacterial diseases and a major impediment to the increase of rice yield. Appropriate methods for inoculation of Xoo and disease scoring are necessary to investigate the nature of the disease and the mechanism of plant ...
    Wheat Coleoptile Inoculation by Fusarium graminearum for Large-scale Phenotypic Analysis
    Authors:  Lei-Jie Jia, Wan-Qiu Wang and Wei-Hua Tang, date: 08/05/2017, view: 5029, Q&A: 0
    [Abstract] The ascomycete fungus Fusarium graminearum is a destructive fungal pathogen of wheat, barley and maize. Although reverse genetics and homologous recombination gene deletion methods have generated thousands of gene deletion mutants of F. graminearum, evaluating virulence of these fungal mutants is still a rate-limiting step. Here ...
    Isolation of Ustilago bromivora Strains from Infected Spikelets through Spore Recovery and Germination
    Authors:  Jason Bosch and Armin Djamei, date: 07/20/2017, view: 3611, Q&A: 0
    [Abstract] Ustilago bromivora is a biotrophic smut fungus infecting Brachypodium sp. It is closely related to the barley-infecting smut Ustilago hordei, and related to the well-studied, gall-inducing model pathogen Ustilago maydis. Upon flowering, the spikelets of U. bromivora-infected plants are filled with black ...
    Exopolysaccharide Quantification for the Plant Pathogen Ralstonia solanacearum
    Authors:  Rémi Peyraud, Timothy P. Denny and Stéphane Genin, date: 05/20/2017, view: 4294, Q&A: 0
    [Abstract] Soluble exopolysaccharide is a major virulence factor produced by the plant pathogen Ralstonia solanacearum. Its massive production during plant infection is associated with the arrest of water flow in xylem vessels leading eventually to plant death. The composition of this heavy macromolecule includes mainly N-acetylgalactosamine. Here ...



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