Plant Science

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    Protocols in Current Issue
    Overrepresentation Analyses of Differentially Expressed Genes in the Smut Fungus Ustilago bromivora during Saprophytic and in planta Growth
    Authors:  Angelika Czedik-Eysenberg, Franziska Rabe, Heinz Ekker, Carmen Czepe and Armin Djamei, date: 08/05/2017, view: 3648, Q&A: 0
    [Abstract] We have established the Ustilago bromivoraBrachypodium spp. interaction as a new model pathosystem for biotrophic fungal plant infections of the head smut type (Rabe et al., 2016). In this protocol, the methodology used for comparing gene expression between saprophytic and in planta growth of the fungus is ...
    An Assay to Study Botrytis cinerea-infected Grapevine Leaves Primed with Pseudomonas fluorescens
    [Abstract] Grapevine (Vitis vinifera L.) is susceptible to an array of diseases among them the grey mold caused by the necrotrophic fungus Botrytis cinerea that decreases grape productivity and quality. To ensure a satisfactory yield and harvest quality numerous chemical fungicides are required, but they have serious drawbacks. One ...
    Cotyledon Wounding of Arabidopsis Seedlings
    Authors:  Debora Gasperini, Ivan F. Acosta and Edward E. Farmer, date: 01/20/2016, view: 6212, Q&A: 0
    [Abstract] Damage to plant organs through both biotic and abiotic injury is very common in nature. Arabidopsis thaliana 5-day-old (5-do) seedlings represent an excellent system in which to study plant responses to mechanical wounding, both at the site of the damage and in distal unharmed tissues. Seedlings of wild type, transgenic or mutant lines ...
    Hyaloperonospora arabidopsidis (Downy Mildew) Infection Assay in Arabidopsis
    Authors:  Shuta Asai, Ken Shirasu and Jonathan D. G. Jones, date: 10/20/2015, view: 8786, Q&A: 2
    [Abstract] Hyaloperonospora arabidopsidis (Hpa; formerly Peronospora parasitica or Hyaloperonospora parasitica) is an oomycete downy mildew pathogen of the model plant Arabidopsis. The pathosystem between Arabidopsis and Hpa has been extensively used to study host/pathogen co-evolution (Coates ...
    Detection of Mitogen-activated Protein Kinase (MAPK) Activation upon Exogenous Chemical Application in Arabidopsis Protoplasts
    Authors:  Arsheed Hussain Sheikh and Alok Krishna Sinha, date: 09/20/2015, view: 6464, Q&A: 0
    [Abstract] The mitogen activated protein kinase cascade is a highly conserved signal pathway in plants. The exogenous chemicals, like hormones, can trigger a series of signalling cascades, including MAPK pathway, to modulate the plant physiology. Upon activation, some MAPKs are phosphorylated. It is important to develop methods that can detect changes in the ...
    An Evaluation of Cellulose Degradation Affected by Dutch Elm Disease
    Authors:  Jaroslav Ďurkovič, František Kačík and Dušan Olčák, date: 07/20/2015, view: 5128, Q&A: 0
    [Abstract] The pathogenic fungus Ophiostoma novo-ulmi spreads within the secondary xylem vessels of infected elm trees, causing the formation of vessel plugs due to tyloses and gels, which ultimately result in Dutch elm disease. Foliage discoloration, wilting and falling from the tree are typical external leaf symptoms of the disease followed by the ...
    A Chemical Genetic Screening Procedure for Arabidopsis thaliana Seedlings
    [Abstract] Unbiased screening approaches are powerful tools enabling identification of novel players in biological processes. Chemical genetic screening refers to the technique of using a reporter response, such as expression of luciferase driven by a promoter of interest, to discover small molecules that affect a given process when applied to plants. These ...
    The Use of a Dexamethasone-inducible System to Synchronize Xa21 Expression to Study Rice Immunity
    Authors:  Daniel F. Caddell, Tong Wei, Chang-Jin Park and Pamela C. Ronald, date: 05/05/2015, view: 7612, Q&A: 0
    [Abstract] Inducible gene expression systems offer researchers the opportunity to synchronize target gene expression at particular developmental stages and in particular tissues. The glucocorticoid receptor (GR), a vertebrate steroid receptor, has been well adopted for this purpose in plants. To generate steroid-inducible plants, a construct of GAL4-binding ...
    A Chemiluminescence Based Receptor-ligand Binding Assay Using Peptide Ligands with an Acridinium Ester Label
    Authors:  Mari Wildhagen, Melinka A. Butenko, Reidunn B. Aalen, Georg Felix and Markus Albert, date: 03/20/2015, view: 6649, Q&A: 0
    [Abstract] Studying the biochemical interaction of ligands with their corresponding receptors requires highly sensitive detection and monitoring of the bound ligand. Classically, radioactively labelled ligands have been widely used as highly sensitive tools for such binding measurements. Disadvantages of radiolabelling include instability of products, high ...
    Airbrush Infiltration Method for Pseudomonas syringae Infection Assays in Soybean
    Authors:  MB Shine, Da-Qi Fu and Aardra Kachroo, date: 03/20/2015, view: 6154, Q&A: 0
    [Abstract] We developed this protocol to assay the extent of proliferation of Pseudomonas syringae pv. glycinea in soybean leaves. This method specifically enables accurate pathogenesis assays of soybean plants at V2/V3 (2nd/3rd trifoliate) or higher stages of growth. The leaves of soybean plants at these growth stages ...



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