Stem Cell

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    Protocols in Current Issue
    Qualitative in vivo Bioluminescence Imaging
    Authors:  Devbarna Sinha, Zalitha Pieterse and Pritinder Kaur, date: 09/20/2018, view: 1662, Q&A: 1
    [Abstract] Bioluminescence imaging (BLI) technology is an advanced method of carrying out molecular imaging on live laboratory animals in vivo. This powerful technique is widely-used in studying a variety of biological processes, and it has been an ideal tool in exploring tumor growth and metastatic spread in real-time. This technique ensures the ...
    Expansion of Airway Basal Cells and Generation of Polarized Epithelium
    Authors:  Hannah Levardon, Lael M. Yonker, Bryan P. Hurley and Hongmei Mou, date: 06/05/2018, view: 2949, Q&A: 1
    [Abstract] Airway basal stem cells are the progenitor cells within the airway that exhibit the capacity to self-renew and give rise to multiple types of differentiated airway epithelial cells. This stem cell-derived epithelium displays organized architecture with functional attributes of the airway mucosa. A protocol has been developed to culture and expand ...
    Hair Follicle Stem Cell Isolation and Expansion
    [Abstract] Stem cells are widely used for numerous clinical applications including limbal stem cell deficiency. Stem cell derived from the bulge region of the hair follicle have the ability to differentiate into a variety of cell types including interfollicular epidermis, hair follicle structures, sebaceous glands and corneal epithelial cells when provided ...
    Murine Hair Follicle Derived Stem Cell Transplantation onto the Cornea Using a Fibrin Carrier
    [Abstract] The goal of this protocol is to establish a procedure for cultivating stem cells on a fibrin carrier to allow for eventual transplantation to the eye. The ability to transfer stem cells to a patient is critical for treatment for a variety of disorders and wound repair. We took hair follicle stem cells from the vibrissae of transgenic mice ...
    Isolation and Culture of Human CD133+ Non-adherent Endothelial Forming Cells
    Authors:  Michaelia P. Cockshell and Claudine S. Bonder, date: 04/05/2016, view: 6336, Q&A: 0
    [Abstract] Circulating endothelial progenitor cells (EPCs) have been the focus of many clinical trials due to their roles in revascularisation following ischemic events such as acute myocardial infarction as well as their contribution to vascular repair during organ transplantation. Research on EPCs has been controversial due to the lack of distinct markers ...
    Isolation and 3-dimensional Culture of Primary Murine Intestinal Epithelial Cells
    Authors:  Agnieszka Pastuła and Michael Quante, date: 05/20/2014, view: 16015, Q&A: 3
    [Abstract] The intestine, together with skin and blood, belongs to the organs with the highest cell turnover, which makes it a perfect model to study cellular processes, such as proliferation and differentiation. Epithelial cell turnover in intestine is possible due to the presence of intestinal stem cells, which are located at the bottom of the crypt. Here, ...



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