Zalitha Pieterse School of Pharmacy & Biomedical Sciences, Faculty Health Curtin Innovation Research Institute, University, Australia,

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Pritinder Kaur School of Pharmacy & Biomedical Sciences, Faculty of Health Sciences, Curtin Health Innovation Research Institute, Curtin University, Australia, Australia,
1 protocol

Devbarna Sinha Research Division, Peter MacCallum Cancer Centre, Australia, Australia,
1 protocol

Zalitha Pieterse

Graduate, School of Pharmacy & Biomedical Sciences, Faculty of Health Sciences, Curtin Health Innovation Research Institute, Curtin University, Australia, Australia,

Research focus

Cell biology
Skin regeneration; Stem cell biology

1 Author merit
Reviewer merit
Editor merit

Education

BSc (Honours), Curtin University, 2016

Lab information

Pritinder Kaur Lab

1 Protocol published

Qualitative in vivo Bioluminescence Imaging

Authors: Devbarna Sinha, Zalitha Pieterse and Pritinder Kaur, date: 09/20/2018, view: 7477, Q&A: 1

Bioluminescence imaging (BLI) technology is an advanced method of carrying out molecular imaging on live laboratory animals in vivo. This powerful technique is widely-used in studying a variety of biological processes, and it has been an ...

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Farrah Aram

None

Hello,

In this protocol, it states that for lentiviral production, the HEK293T cells are transfected using FuGENE 6 and the FUGLW plasmid. May you clarify at which step the packaging and envelope vectors are added?

3/21/2019 11:59:02 AM Reply

Farrah Aram

None

Hello,

Thank you for your reply to my previous question. I do, however, have an another one. Can you suggest any commercially available alternatives to the FULGW plasmid used in this protocol? Thank you in advance.

Sincerely,
Farrah

2019-03-31 07:09:26

Pritinder Kaur

School of Pharmacy & Biomedical Sciences, Faculty of Health Sciences, Curtin Health Innovation Research Institute, Curtin University, Australia, Australia,

Hi Farrah

Apologies for not making this clear in the protocol. The HEK293 cells are co-transfected with FUGLW AND the packaging vectors to generate replication-defective lentivirus. We cited Sinha et al., 2016 which explains this methodology a bit better.

Sincerely
Pritinder kaur

2019-03-24 19:20:18