Stem Cell

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    Protocols in Current Issue
    3D Organoid Formation from the Murine Salivary Gland Cell Line SIMS
    Authors:  Harleen K. Athwal and Isabelle M. A. Lombaert, date: 10/05/2019, view: 1834, Q&A: 0
    [Abstract] Salivary glands consist of multiple phenotypically and functionally unique cell populations, such as the acinar, ductal, and myoepithelial cells that help produce, modify, and secrete saliva (Lombaert et al., 2011). Identification of mechanisms and factors that regulate these populations has been of key interest, as salivary gland-related ...
    Maintenance of Schmidtea mediterranea in the Laboratory
    Authors:  Nídia de Sousa and Teresa Adell, date: 10/05/2018, view: 3035, Q&A: 0
    [Abstract] In the last years, planarians have emerged as a unique model animal for studying regeneration and stem cells biology. Although their remarkable regenerative abilities are known for a long time, only recently the molecular tools to understand the biology of planarian stem cells and the fundamentals of their regenerative process have been ...
    Isolation and Expansion of Mesenchymal Stem Cells from Murine Adipose Tissue
    Authors:  Natalia V. Andreeva, Alexandra A. Dalina and Alexander V. Belyavsky, date: 08/20/2017, view: 7735, Q&A: 1
    [Abstract] Mesenchymal stem cells (MSCs) are currently intensively studied due to significant promise which they represent for successful implementations of future cell therapy clinical protocols. This in turn emphasizes importance of careful preclinical studies of MSC effects in various murine disease models. The appropriate cell preparations with ...
    Functional Analysis of Connexin Channels in Cultured Cells by Neurobiotin Injection and Visualization
    Authors:  Philipp Wörsdörfer and Klaus Willecke, date: 06/05/2017, view: 5404, Q&A: 0
    [Abstract] Functional gap junction channels between neighboring cells can be assessed by microinjection of low molecular weight tracer substances into cultured cells. The extent of direct intercellular communication can be precisely quantified by this method. This protocol describes the iontophoretic injection and visualisation of Neurobiotin into cultured ...
    A Streamlined Method for the Preparation of Growth Factor-enriched Thermosensitive Hydrogels from Soft Tissue
    [Abstract] Hydrogels are an ideal medium for the expansion of cells in three dimensions. The ability to induce cell expansion and differentiation in a controlled manner is a key goal in tissue engineering. Here we describe a detailed method for producing hydrogels from soft tissues with an emphasis on adipose tissue. In this method, soluble, extractable ...
    Ex vivo Culture of Fetal Mouse Gastric Epithelial Progenitors
    [Abstract] Isolation and tridimensional culture of murine fetal progenitors from the digestive tract represents a new approach to study the nature and the biological characteristics of these epithelial cells that are present before the onset of the cytodifferentiation process during development. In 2013, Mustata et al. described the isolation of ...
    Ex vivo Culture of Adult Mouse Antral Glands
    [Abstract] The tri-dimensional culture, initially described by Sato et al. (2009) in order to isolate and characterize epithelial stem cells of the adult small intestine, has been subsequently adapted to many different organs. One of the first examples was the isolation and culture of antral stem cells by Barker et al. (2010), who ...
    Pit Assay to Measure the Bone Resorptive Activity of Bone Marrow-derived Osteoclasts
    Authors:  Alexander Vesprey and Wentian Yang, date: 06/20/2016, view: 9144, Q&A: 0
    [Abstract] Although it is possible to use a tartrate-resistant acid phosphatase (TRAP) stain to assist in identifying osteoclasts, a separate method is needed to determine the bone resorption activity of osteoclasts. Since osteoclasts leave “pits” after bone matrix resorption (Charles et al., 2014), it is possible to stain pits as a method of ...
    Porous Scaffold Seeding and Chondrogenic Differentiation of BMSC-seeded Scaffolds
    Authors:  Troy D. Bornes, Nadr M. Jomha, Aillette Mulet-Sierra and Adetola B. Adesida, date: 12/20/2015, view: 6407, Q&A: 0
    [Abstract] Bone marrow-derived mesenchymal stromal stem cells (BMSCs) are a promising cell source for treating articular cartilage defects (Bornes et al., 2014). BMSCs can be seeded within porous biomaterial scaffolds that support three-dimensional cell organization, chondrogenic differentiation and extracellular matrix deposition for the creation ...
    Skeletal Myogenesis in vitro
    Authors:  William C. W. Chen and Bruno Péault, date: 11/05/2015, view: 6875, Q&A: 0
    [Abstract] Mature skeletal myofibers are elongated and multinucleated cells. Many stem/progenitor cell types, including committed muscle stem (satellite cells) and progenitor (myoblasts) cells, muscle-derived stem cells, myogenic endothelial cells, and mesenchymal stem/stromal cells, have been shown to exhibit skeletal myogenesis under appropriate inductive ...



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