Maintenance of Schmidtea mediterranea in the Laboratory
Authors: Nídia de Sousa and
Teresa Adell,
date: 10/05/2018,
view: 1355,
Q&A: 0
[Abstract] In the last years, planarians have emerged as a unique model animal for studying regeneration and stem cells biology. Although their remarkable regenerative abilities are known for a long time, only recently the molecular tools to understand the biology of planarian stem cells and the fundamentals of their regenerative process have been ...
Isolation and Expansion of Mesenchymal Stem Cells from Murine Adipose Tissue
[Abstract] Mesenchymal stem cells (MSCs) are currently intensively studied due to significant promise which they represent for successful implementations of future cell therapy clinical protocols. This in turn emphasizes importance of careful preclinical studies of MSC effects in various murine disease models. The appropriate cell preparations with ...
Ex vivo Culture of Fetal Mouse Gastric Epithelial Progenitors
[Abstract] Isolation and tridimensional culture of murine fetal progenitors from the digestive tract represents a new approach to study the nature and the biological characteristics of these epithelial cells that are present before the onset of the cytodifferentiation process during development. In 2013, Mustata et al. described the isolation of ...
Ex vivo Culture of Adult Mouse Antral Glands
[Abstract] The tri-dimensional culture, initially described by Sato et al. (2009) in order to isolate and characterize epithelial stem cells of the adult small intestine, has been subsequently adapted to many different organs. One of the first examples was the isolation and culture of antral stem cells by Barker et al. (2010), who ...
Porous Scaffold Seeding and Chondrogenic Differentiation of BMSC-seeded Scaffolds
[Abstract] Bone marrow-derived mesenchymal stromal stem cells (BMSCs) are a promising cell source for treating articular cartilage defects (Bornes et al., 2014). BMSCs can be seeded within porous biomaterial scaffolds that support three-dimensional cell organization, chondrogenic differentiation and extracellular matrix deposition for the creation ...
Skeletal Myogenesis in vitro
Authors: William C. W. Chen and
Bruno Péault,
date: 11/05/2015,
view: 5604,
Q&A: 0
[Abstract] Mature skeletal myofibers are elongated and multinucleated cells. Many stem/progenitor cell types, including committed muscle stem (satellite cells) and progenitor (myoblasts) cells, muscle-derived stem cells, myogenic endothelial cells, and mesenchymal stem/stromal cells, have been shown to exhibit skeletal myogenesis under appropriate inductive ...
Clonal Culture of Mouse Liver Progenitor Cells
Author: Naoki Tanimizu,
date: 10/20/2015,
view: 7142,
Q&A: 0
[Abstract] Liver stem/progenitor cells (LPCs) are defined as bipotential cells differentiating into both hepatocytes and cholangiocytes. For analyzing their differentiation potential, clonal culture has been used for LPCs isolated by a cell sorter. In addition, we can use the culture to assess functions of target genes on differentiation potential of LPCs. ...