Cancer Biology

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    Protocols in Current Issue
    Adoptive Transfer of Monocytes Sorted from Bone Marrow
    Authors:  Damya Laoui, Eva Van Overmeire, Chloé Abels, Jiri Keirsse and Jo A Van Ginderachter, date: 01/05/2019, view: 202, Q&A: 0
    [Abstract] Inflammatory Ly6Chi monocytes can give rise to distinct mononuclear myeloid cells in the tumor microenvironment, such as monocytic myeloid-derived suppressor cells (Mo-MDSC), immature macrophages, M2-like tumor-associated macrophages (TAMs), M1-like TAMs or monocyte-derived dendritic cells (Mo-DCs). This protocol describes a method to ...
    In-vivo gp100-specific Cytotoxic CD8+ T Cell Killing Assay
    [Abstract] Cytotoxic CD8+ T lymphocytes (CTLs) represent a crucial component of the adaptive immune system and play a prominent role in the anti-tumor immune responses of both mice and humans.

    Cytotoxic CD8+ T cells are responsible for the lysis of cells expressing peptides associated with MHC class I molecules and derived ...
    3D Co-culture System of Tumor-associated Macrophages and Ovarian Cancer Cells
    Authors:  Lingli Long, Mingzhu Yin and Wang Min, date: 04/20/2018, view: 2121, Q&A: 0
    [Abstract] Ovarian cancer is fairly unique in that ovarian carcinoma cells can detach and spread directly through peritoneal cavity. It has been unclear, however, how detached cancer cells survive in the peritoneum and form spheroid structure. We have recently reported that there is a strong correlation between Tumor-associated macrophages (TAMs)-associated ...
    Glioma Induction by Intracerebral Retrovirus Injection
    Authors:  Ravinder K Verma , Fanghui Lu and Qing Richard Lu, date: 07/20/2017, view: 3319, Q&A: 0
    [Abstract] Glioblastoma (GBM) is the most common primary brain cancer in adults and has a poor prognosis. It is characterized by a high degree of cellular infiltration that leads to tumor recurrence, atypical hyperplasia, necrosis, and angiogenesis. Despite aggressive treatment modalities, current therapies are ineffective for GBM. Mouse GBM models not only ...
    In vivo Efficacy Studies in Cell Line and Patient-derived Xenograft Mouse Models
    Authors:  Elizabeth A. Tovar, Curt J. Essenburg and Carrie Graveel, date: 01/05/2017, view: 5887, Q&A: 0
    [Abstract] In vivo xenograft models derived from human cancer cells have been a gold standard for evaluating the genetic drivers of cancer and are valuable preclinical models for evaluating the efficacy of cancer therapeutics. Recently, patient-derived tumorgrafts from multiple tumor types have been developed and shown to more accurately ...
    Quantification of Tumor Material Uptake
    Authors:  Richard N. Hanna and Catherine C. Hedrick, date: 10/20/2016, view: 3942, Q&A: 0
    [Abstract] Extracellular tumor material including exosomes, microvesicles and apoptotic tumor debris may help cancers invade new organs. Enhancing the removal of extracellular tumor material by immune cells represents a novel immunotherapy approach for preventing cancer metastasis. This protocol quantifies the uptake and removal of extracellular tumor ...
    In vivo Imaging of Tumor and Immune Cell Interactions in the Lung
    Authors:  Richard N. Hanna, Grzegorz Chodaczek and Catherine C. Hedrick, date: 10/20/2016, view: 3891, Q&A: 0
    [Abstract] Immunotherapy has demonstrated great therapeutic potential by activating the immune system to fight cancer. However, little is known about the specific dynamics of interactions that occur between tumor and immune cells. In this protocol we describe a novel method to visualize the interaction of tumor and immune cells in the lung of live mice, ...
    Peptide Loading on MHC Class I Molecules of Tumor Cells
    Authors:  Loredana Cifaldi, Franco Locatelli and Doriana Fruci, date: 09/20/2016, view: 4336, Q&A: 0
    [Abstract] MHC class I molecules present peptides to cytotoxic T cells allowing the immune system to scan for intracellular pathogens and mutated proteins. The generation of antigenic peptides is a multistep process that ends in the endoplasmic reticulum (ER). Only peptides with the right length and sequence will bind nascent MHC class I molecules in the ER. ...
    Killer Cell Ig-like Receptors (KIR)-Binding Assay for Tumor Cells
    Authors:  Loredana Cifaldi, Franco Locatelli and Doriana Fruci, date: 09/20/2016, view: 2846, Q&A: 0
    [Abstract] Natural killer (NK) cells play key roles in innate and adaptive immune responses against virus and tumor cells. Their function relies on the dynamic balance between activating and inhibiting signals through receptors that bind ligands expressed on target cells. The absence of inhibitory receptor engagement with their ligands and the presence of ...
    Macrophage Polarization by Tumor-induced MDSCs Assay
    Authors:  Felipe Vences-Catalán, Minu K. Srivastava and Shoshana Levy, date: 08/20/2016, view: 5652, Q&A: 0
    [Abstract] Myeloid derived suppressor cells (MDSCs) are a subset of granulocytes (immature myeloid cells) that exploit a variety of mechanism to modulate the innate and adaptive immune system. MDSCs are present normally in the body, but their numbers increase during inflammation and in cancer, promoting an immunosuppressive microenvironment. In addition to ...



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