Cancer Biology


    Protocols in Current Issue
    Myeloid Progenitor Transformation Assay
    Authors:  Hiroshi Okuda and Akihiko Yokoyama, date: 12/05/2017, view: 4449, Q&A: 0
    [Abstract] Numerous oncogenes have been identified to cause leukemia. For example, chromosomal translocation generates various fusion genes of the mixed-lineage leukemia (MLL) gene and a partner gene in leukemia, whose gene products transform primary myeloid progenitors into an immortalized state. To characterize the transforming ability of leukemic ...
    Telomere Restriction Fragment (TRF) Analysis
    Authors:  Ilgen Mender and Jerry W. Shay, date: 11/20/2015, view: 15030, Q&A: 0
    [Abstract] While telomerase is expressed in ~90% of primary human tumors, most somatic tissue cells except transiently proliferating stem-like cells do not have detectable telomerase activity (Shay and Wright, 1996; Shay and Wright, 2001). Telomeres progressively shorten with each cell division in normal cells, including proliferating stem-like cells, due to ...
    Telomerase Repeated Amplification Protocol (TRAP)
    Authors:  Ilgen Mender and Jerry W. Shay, date: 11/20/2015, view: 13878, Q&A: 1
    [Abstract] Telomeres are found at the end of eukaryotic linear chromosomes, and proteins that bind to telomeres protect DNA from being recognized as double-strand breaks thus preventing end-to-end fusions (Griffith et al., 1999). However, due to the end replication problem and other factors such as oxidative damage, the limited life span of cultured ...
    Telomere Dysfunction Induced Foci (TIF) Analysis
    Authors:  Ilgen Mender and Jerry W. Shay, date: 11/20/2015, view: 7806, Q&A: 0
    [Abstract] Telomerase maintains telomeric DNA in eukaryotes during early developments, ~90% of cancer cells and some proliferative stem like cells. Telomeric repeats at the end of chromosomes are associated with the shelterin complex. This complex consists of TRF1, TRF2, Rap1, TIN2, TPP1, POT1 which protect DNA from being recognized as DNA double-stranded ...
    Detection of ALT Associated Promyelocytic Leukemia Nuclear Bodies (APBs) by Immunofluorescence-FISH (IF-FISH)
    Authors:  Siamak A. Kamranvar and Maria G. Masucci, date: 12/05/2014, view: 9896, Q&A: 0
    [Abstract] The activation of functions that counteract the physiological shortening of telomeres in rapidly proliferating cell is prerequisite for the progression of cancer cells to full malignancy (Collado et al., 2007). In most human cancers, the length of telomere is maintained through up-regulation of telomerase whereas a telomerase-independent ...

    We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.