RNA Stability Measurements Using RT-qPCR in Arabidopsis Seedlings
Authors: Tianran Jia and
Brandon H. Le,
date: 07/20/2020,
view: 700,
Q&A: 0
[Abstract] Steady-state mRNA levels are determined by both the rates of transcription and degradation. Regulation of mRNA stability and/or degradation are key factors that can significantly affect mRNA levels and its biological functions. mRNA stability can be measured indirectly after transcription inhibition. This protocol described a rapid and sensitive ...
Methylation-sensitive Amplified Polymorphism as a Tool to Analyze Wild Potato Hybrids
[Abstract] Methylation-Sensitive Amplification Polymorphism (MSAP) is a versatile marker for analyzing DNA methylation patterns in non-model species. The implementation of this technique does not require a reference genome and makes it possible to determine the methylation status of hundreds of anonymous loci distributed throughout the genome. In addition, ...
Safe DNA-extraction Protocol Suitable for Studying Tree-fungus Interactions
[Abstract] We present a safe and low-cost method suitable for DNA extraction from mycelium and tree tissue samples. After sample preparation, the extraction takes about 60 min. Method performance was tested by extracting DNA from various tree tissue samples and from mycelium grown on solid and liquid media. DNA was extracted from juvenile and mature host ...
Measuring Protein Half-life in Arabidopsis thaliana
Authors: Hongqing Guo and
Yanhai Yin,
date: 08/05/2019,
view: 2008,
Q&A: 0
[Abstract] Post-translational modifications play important roles in controlling protein function and can lead to altered protein stability. Protein stability can be determined after treatment with the protein synthesis inhibitor Cycloheximide. Cycloheximide is a translational inhibitor that inhibits protein synthesis via cytoplasmic ribosomes. Here we ...
Extraction and Purification of Laccases from Rice Stems
Authors: Chenna Swetha and
P. V. Shivaprasad,
date: 04/05/2019,
view: 2901,
Q&A: 0
[Abstract] Laccases are found in cell walls of plants in very low amounts. This protocol provides an efficient method to purify laccases from rice stems. The method involves three steps: 1) Isolation of total protein from rice stems using buffers with high salt concentration to extract protein from cell walls; 2) Purification of laccases using concanavalin-A ...