Lili Jing Department of Cell and Molecular Biology, University Pennsylvania, USA

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Lili Jing

Department of Cell and Molecular Biology, University of Pennsylvania, USA

Research focus

Molecular biology

17 Author merit
Reviewer merit
Editor merit

17 Protocols published

Cell Cycle Analysis and GFP Expression of Zebrafish Embryos by FACS

Author: Lili Jing, date: 02/20/2012, view: 16820, Q&A: 0

Cell cycle analysis in combination of GFP expression has been well used to study the cell cycle distribution of GFP labeled cells. This protocol is a method to analyze the cell cycles of GFP marked cells from zebrafish embryos.

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Zebrafish Embryo DNA Preparation

Author: Lili Jing, date: 02/20/2012, view: 20868, Q&A: 1

This protocol explains how to extract DNA from a single zebrafish embryo. It does not require the use of expensive kits.

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Özge Çark

CRTD

Hi,

I tried your protocol to extract gDNA from a single embryo for mutation screening. Although my primers are specific for the target region at 60C annealing temp in my other PCRs, when I use gDNA from this protocol, I obtained nonspecific bands. What could be happened in the reaction? Can I fix this problem by adjusting annealing temperature?

Best regards,

Özge

7/12/2019 4:27:10 AM Reply

Özge Çark

CRTD

Thank you :) I will try to adjust annealing temp.

2019-07-16 04:22:42

ajay verma

centre for cellular and molecular biology

Hi,
This happened with one of my primer pair too but worked by adjusting the annealing temperature. It might be due to high salts.
Good luck,
Ajay

2019-07-16 00:39:38

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Lili Jing

Department of Cell and Molecular Biology, University of Pennsylvania, USA

Özge Çark

CRTD

Özge Çark

CRTD

ajay verma

centre for cellular and molecular biology