Education

Dr. rer. nat, University Tübingen, Germany, 1980

Current position

Senior Scientist, Institut für Industrielle Genetik, Universität Stuttgart, Germany

Publications

1. Dobruchowska, J. M., Jonsson, J. O., Fridjonsson, O. H., Aevarsson, A., Kristjansson, J. K., Altenbuchner, J., Watzlawick, H., Gerwig, G. J., Dijkhuizen, L., Kamerling, J. P. and Hreggvidsson, G. O. (2016). Modification of linear (beta1-->3)-linked gluco-oligosaccharides with a novel recombinant beta-glucosyltransferase (trans-beta-glucosidase) enzyme from Bradyrhizobium diazoefficiens. Glycobiology 26(11): 1157-1170.
2. Watzlawick, H., Morabbi Heravi, K. and Altenbuchner, J. (2016). Role of the ganSPQAB Operon in Degradation of Galactan by Bacillus subtilis. J Bacteriol 198(20): 2887-2896.
3. Tolzer, C., Pal, S., Watzlawick, H., Altenbuchner, J. and Niefind, K. (2016). A novel esterase subfamily with alpha/beta-hydrolase fold suggested by structures of two bacterial enzymes homologous to L-homoserine O-acetyl transferases. FEBS Lett 590(1): 174-184.
4. Wang, L., Hoffmann, J., Watzlawick, H. and Altenbuchner, J. (2015). Markerless Gene Deletion with Cytosine Deaminase in Thermus thermophilus Strain HB27. Appl Environ Microbiol 82(4): 1249-1255.
5. Heravi, K. M., Watzlawick, H. and Altenbuchner, J. (2015). Development of an anhydrotetracycline-inducible expression system for expression of a neopullulanase in B. subtilis. Plasmid 82: 35-42.
6. Morabbi Heravi, K., Lange, J., Watzlawick, H., Kalinowski, J. and Altenbuchner, J. (2015). Transcriptional regulation of the vanillate utilization genes (vanABK Operon) of Corynebacterium glutamicum by VanR, a PadR-like repressor. J Bacteriol 197(5): 959-972.
7. Wang, L., Watzlawick, H., Fridjonsson, O., Hreggvidsson, G. and Altenbuchner, J. (2013). Improved soluble expression of the gene encoding amylolytic enzyme Amo45 by fusion with the mobile-loop-region of co-chaperonin GroES in Escherichia coli. Biocatal Biotransformation 31(6): 335-342.
8. Lipski, A., Watzlawick, H., Ravaud, S., Robert, X., Rhimi, M., Haser, R., Mattes, R. and Aghajari, N. (2013). Mutations inducing an active-site aperture in Rhizobium sp. sucrose isomerase confer hydrolytic activity. Acta Crystallogr D Biol Crystallogr 69(Pt 2): 298-307.
9. Merceron, R., Foucault, M., Haser, R., Mattes, R., Watzlawick, H. and Gouet, P. (2012). The molecular mechanism of thermostable alpha-galactosidases AgaA and AgaB explained by x-ray crystallography and mutational studies. J Biol Chem 287(47): 39642-39652.
10. Watzlawick, H. and Mattes, R. (2009). Gene cloning, protein characterization, and alteration of product selectivity for the trehalulose hydrolase and trehalulose synthase from "Pseudomonas mesoacidophila" MX-45. Appl Environ Microbiol 75(22): 7026-7036.
11. Placier, G., Watzlawick, H., Rabiller, C. and Mattes, R. (2009). Evolved beta-galactosidases from Geobacillus stearothermophilus with improved transgalactosylation yield for galacto-oligosaccharide production. Appl Environ Microbiol 75(19): 6312-6321.
12. Ravaud, S., Robert, X., Watzlawick, H., Haser, R., Mattes, R. and Aghajari, N. (2009). Structural determinants of product specificity of sucrose isomerases. FEBS Lett 583(12): 1964-1968.
13. Tolzer, C., Pal, S., Watzlawick, H., Altenbuchner, J. and Niefind, K. (2009). Crystallization and preliminary crystallographic analysis of cgHle, a homoserine acetyltransferase homologue, from Corynebacterium glutamicum. Acta Crystallogr Sect F Struct Biol Cryst Commun 65(Pt 1): 34-38.
14. Ravaud, S., Robert, X., Watzlawick, H., Haser, R., Mattes, R. and Aghajari, N. (2007). Trehalulose synthase native and carbohydrate complexed structures provide insights into sucrose isomerization. J Biol Chem 282(38): 28126-28136.
15. Foucault, M., Watzlawick, H., Mattes, R., Haser, R. and Gouet, P. (2006). Crystallization and preliminary X-ray diffraction studies of two thermostable alpha-galactosidases from glycoside hydrolase family 36. Acta Crystallogr Sect F Struct Biol Cryst Commun 62(Pt 2): 100-103.
16. Ravaud, S., Watzlawick, H., Haser, R., Mattes, R. and Aghajari, N. (2006). Overexpression, purification, crystallization and preliminary diffraction studies of the Protaminobacter rubrum sucrose isomerase SmuA. Acta Crystallogr Sect F Struct Biol Cryst Commun 62(Pt 1): 74-76.
17. Ravaud, S., Watzlawick, H., Haser, R., Mattes, R. and Aghajari, N. (2005). Expression, purification, crystallization and preliminary X-ray crystallographic studies of the trehalulose synthase MutB from Pseudomonas mesoacidophila MX-45. Acta Crystallogr Sect F Struct Biol Cryst Commun 61(Pt 1): 100-103.
18. Fridjonsson, O., Watzlawick, H. and Mattes, R. (2002). Thermoadaptation of alpha-galactosidase AgaB1 in Thermus thermophilus. J Bacteriol 184(12): 3385-3391.
19. Fridjonsson, O., Watzlawick, H. and Mattes, R. (2000). The structure of the alpha-galactosidase gene loci in Thermus brockianus ITI360 and Thermus thermophilus TH125. Extremophiles 4(1): 23-33.
20. Fridjonsson, O., Watzlawick, H., Gehweiler, A., Rohrhirsch, T. and Mattes, R. (1999). Cloning of the gene encoding a novel thermostable alpha-galactosidase from Thermus brockianus ITI360. Appl Environ Microbiol 65(9): 3955-3963.
21. Fridjonsson, O., Watzlawick, H., Gehweiler, A. and Mattes, R. (1999). Thermostable alpha-galactosidase from Bacillus stearothermophilus NUB3621: cloning, sequencing and characterization. FEMS Microbiol Lett 176(1): 147-153.
22. Watzlawick, H., Walsh, M. T., Yoshioka, Y., Schmid, K. and Brossmer, R. (1992). Structure of the N- and O-glycans of the A-chain of human plasma alpha 2HS-glycoprotein as deduced from the chemical compositions of the derivatives prepared by stepwise degradation with exoglycosidases. Biochemistry 31(48): 12198-12203.
23. Watzlawick, H., Walsh, M. T., Ehrhard, I., Slayter, H. S., Haupt, H., Schwick, H. G., Jourdian, G. W., Hase, S., Schmid, K. and Brossmer, R. (1991). The effect of the carbohydrate moiety upon the size and conformation of human plasma galactoglycoprotein as judged by electron microscopy and circular dichroism. Structural studies of a glycoprotein after stepwise enzymic carbohydrate removal. Biochem J 277 ( Pt 3): 753-758.
24. Walsh, M. T., Watzlawick, H., Putnam, F. W., Schmid, K. and Brossmer, R. (1990). Effect of the carbohydrate moiety on the secondary structure of beta 2-glycoprotein. I. Implications for the biosynthesis and folding of glycoproteins. Biochemistry 29(26): 6250-6257.
25. Gross, H. J., Kovac, A., Rose, U., Watzlawick, H. and Brossmer, R. (1988). Interaction of N-acetyl-4-epi-D-neuraminic acid with key enzymes of sialic acid metabolism. Biochemistry 27(12): 4279-4283.
26. Schott, H., Schrade, H. and Watzlawick, H. (1984). Isolation of oligonucleotides from partial hydrolysates of DNA using template chromatography. J Chromatogr 285(2): 343-363.