Fanglian He Bio-protocol
205 protocols

Hui Zhu Stanford University
18 protocols

Kate Hannan The Australian National University
15 protocols

Omar Akil University of California, San Francisco
18 protocols

Lin Fang
  • Stanford University
Research focus
  • Cell biology
  • 4 Author merit


PhD, University of Oregon, 2016

4 Protocols published
Antibody Purification from Western Blotting
Author:  Lin Fang, date: 03/20/2012, view: 21690, Q&A: 2
This protocol describes a method of purifying antibodies from sera with denatured antigens immobilized on western blot membranes. Advantages include (1) fast and easy; (2) purification of antibody with antigen in denatured form allows high yield in ...
Expression and Purification of GST-tagged Proteins from E. coli
Author:  Lin Fang, date: 09/20/2011, view: 21455, Q&A: 1
This protocol describes a method for the small and large-scale expression and purification of GST proteins. Due to the diverse nature of proteins, a small-scale expression and purification test is always recommended.
18 Protocols reviewed
Actin Retrograde Flow in Permeabilized Cells: Myosin-II Driven Centripetal Movement of Transverse Arcs
Authors:  Yee Han Tee and Alexander D. Bershadsky, date: 03/05/2016, view: 8259, Q&A: 0
Numerous biological functions such as cytokinesis, changes in cell shape and cell migration require actomyosin-driven cellular contractility. However, the detailed mechanism of how contractile forces drive cellular processes are difficult to ...
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Three Dimensional Spheroid Co-culture Invasion Assay
Authors:  Eldo T Verghese and Thomas A Hughes, date: 01/05/2015, view: 12376, Q&A: 0
The assay was developed to investigate the impact of stromal cells of different types (in our case breast cancer associated fibroblasts stably manipulated to modify expression of genes of interest) on the invasive capacity of epithelial cancer cells ...
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77 Protocols edited
Preparation of Golgi Membranes from Rat Liver
Authors:  Carmen Valente, Gabriele Turacchio, Stefania Spanò, Alberto Luini and Daniela Corda, date: 11/20/2014, view: 8322, Q&A: 0
This protocol details the isolation of enriched Golgi membranes from rat liver, using discontinuous density gradient centrifugation. This high-yield extraction method is useful for several applications, including immunoprecipitation of solubilised ...
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Lysosomal Stability Assay
Authors:  Nikolaj H. T. Petersen, Thomas Kirkegaard and Marja Jäättelä, date: 06/20/2014, view: 16236, Q&A: 0
This assay makes use of the dye Acridine Orange (AO) to determine the stability of lysosomes in living cells upon exposure to a confocal microscope laser.

AO is a lipophilic amine that readily diffuses into cells (Figure 1). Inside the ...
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