Improve Research Reproducibility A Bio-protocol resource

SL
Susan Lauw
  • Post-Doc, Core Facility Signalling Factory & Robotics, University of Freiburg, Germany
Research fields
  • Biochemistry, Biophysics, Cell Biology, Microbiology, Plant Science
Personal information

Education

Dr. Rer. Nat, Technische Universität München, 2008

Lab information

Core Facility Signalling Factory & Robotics
https://uni-freiburg.de/signalling-factory-robotics/

Research focus

Automation (High throughput screening)
Operator of Tecan Freedom Evo
Assay development, optimisation
Enzyme characterisation
Single cell genomics

Publications

Rohdich, F., Lauw, S., Kaiser, J., Feicht, R., Kohler, P., Bacher, A., Eisenreich, W. (2006). Isoprenoid biosynthesis in plants – 2C-methyl-D-erythritol-4-phosphate synthase (IspC protein) of Arabidopsis Thaliana. FEBS Journal 273 (2006) 4446–4458 ª 2006. https://doi.org/10.1111/j.1742-4658.2006.05446.x

Crane, C. M., Kaiser, J., Ramsden, N. L., Lauw, S., Rohdich, F., Eisenreich, W., Hunter, W. N., Bacher, A., and Diederich, F. (2006). Fluorescent Inhibitors for IspF, an Enzyme in the Non-Mevalonate Pathway for Isoprenoid Biosynthesis and a Potential Target for Antimalarial Therapy, Angew. Chem. Int. Ed. 2006, 45, 1069 –1074. https://doi.org/10.1002/anie.200503003

Kaiser, J., Yassinb, M., Prakash, S., Safib, N., Agamid, M., Lauw, S., Ostrozhenkovaa, E., Bacher, A., Rohdich, F., Eisenreich, W., Safi, J., Golan- Goldhirshc, A. (2007). Anti-malarial drug targets: Screening for inhibitors of 2C-methyl-D-erythritol 4- phosphate synthase (IspC protein) in Mediterranean plants.Phytomedicine April 2007, Vol 14, Issue 4, 242-249. https://doi.org/10.1016/j.phymed.2006.12.018

Hirsch, A. K. H., Lauw, S., Gersbach, P., Schweizer, W. B., Rohdich, F., Eisenreich, W., Bacher, A., and Diederich, F. (2007). Nonphosphate inhibitors of IspE protein, a kinase in the non-mevalonate pathway for isoprenoid biosynthesis and a potential target for antimalarial therapy, ChemMedChem March 2007, Vol.2, Issue 6, 806-810. https://doi.org/10.1002/cmdc.200700014

Baumgartner, C., Eberle, C., Diederich, C., Lauw, S., Rohdich, F., Eisenreich, W., Bacher, A. (2007). Structure-Based Design and Synthesis of the First Weak Non-Phosphate Inhibitors for IspF, an Enzyme in the Non-Mevalonate Pathway of Isoprenoid Biosynthesis; Helvetica Chimica Acta, June 2007, Volume 90, Issue 6 , Pages 1043 – 1068. https://doi.org/10.1002/hlca.200790105

Crane, C. M., Hirsch, A. K. H., Alphey, M. S., Sgraja, T., Lauw, S., Illarionova, V., Rohdich, F., Eisenreich, W., Hunter, W. N., Bacher, A., and Diederich, F. (2008) Synthesis and Characterization of Cytidine Derivatives that Inhibit the Kinase IspE of the Non-Mevalonate Pathway for Isoprenoid Biosynthesis, ChemMedChem 2008, 3, 91 – 101. https://doi.org/10.1002/cmdc.200700208

Sgraja, T., Alphey, M. S., Ghilagaber, S. Marquez, R., Robertson, M. N., Hemmings, J. L., Lauw, S., Rohdich, F., Bacher, A., Eisenreich, W., Illarionova, V. and Hunter, W. N. (2008). Characterization of Aquifex aeolicus 4-diphosphocytidyl- 2C-methyl-D-erythritol kinase – ligand recognition in a template for antimicrobial drug discovery, FEBS Journal 2008, The Authors Journal compilation 2008 FEBS. https://doi.org/10.1111/j.1742-4658.2008.06418.x


Hirsch, A. K. H., Alphey, M. S., Lauw, S., Seet, M., Barandun, L., Eisenreich, W., Rohdich, F., Hunter, W. N., Bacher, A. And Diederich, F. (2008). Inhibitors of the kinase IspE: structure–activity relationships and co-crystal structure analysis, Org. Biomol. Chem., 2008. https://doi.org/10.1039/B804375B

Lauw, S., Illarionova, V., Bacher, A., Rohdich, F., and Eisenreich, W. (2008). Biosynthesis of isoprenoids - studies on the mechanism of 2Cmethyl-d-erythritol-4-phosphate synthase, FEBS Journal, 2008. https://doi.org/10.1111/j.1742-4658.2008.06547.x

Geist, J. G., Lauw, S., Illarionova, V., Illarionov, B., Fischer, M., Gräwert, T., Rohdich, F., Eisenreich, W., Kaiser, J., Groll, M., Scheurer, C., Wittlin, S., Alonso-Gómez, J. L., Schweizer, W. B., Bacher, A. and Diederich, F. (2010). Thiazolopyrimidine Inhibitors of 2-Methylerythritol 2,4-Cyclodiphosphate Synthase (IspF) from Mycobacterium tuberculosis and Plasmodium falciparum, ChemMedChem 2010, 5, 1092 – 1101. https://doi.org/10.1002/cmdc.201000083

Lan, C., Stulz, A., Barthes, N. P. F., Lauw, S., Salavei, P., Jung, M., Heerklotz, H., and Ulbrich, M. H. (2021). Designed membrane protein heterodimers and control of their affinity by binding domain and membrane linker properties, Nanoscale, 2021, 13, 20692–20702. https://doi.org/10.1039/D1NR06574B

Wolf, I., Storz, J., Schultze-Seemann, S., Esser, P. R., Martin, S. F., Lauw, S., Fischer, P., Peschers, M., Melchinger, M., Zeiser, R., Gorka, O., Groß, O., Gratzke, C., Brückner, R., Wolf, P. (2004). A new silicon phthalocyanine dye induces pyroptosis in prostate cancer cells during photoimmunotherapy, Bioactive Materials 41 (2024) 537–552. https://doi.org/10.1016/j.bioactmat.2024.07.025

Rößling, A.-K., Dünser, K., Liu, C., Lauw, S., Rodriguez- Franco, M., Kalmbach, L., Barbez, E., Kleine- Vehn, J. (2004). Pectin methylesterase activity is required
for RALF1 peptide signalling output, eLife 2024;13:RP96943. https://doi.org/10.7554/eLife.96943.3

Waibel, S., Graf, N., Storz, J., Schultze-Seemann, S., Lauw, S., Gratzke, C., Brückne, R., Wolf, P. and Wolf, S. (2004). Photoimmunotherapy of Prostate Cancer WithAntibody and Fab Fragments Targeting the Prostate Specific Membrane Antigen, ANTICANCER RESEARCH 44: 2343-2348 (2024). https://doi.org/10.21873/anticanres.17041

Huber, F., Wolf, I., Storz, J., Schultze-Seemann, S., Lauw, S., Klemenz, L., Miernik, A., Gratzke, C., Brückner, R., Wolf, P. (2024). Antibody-dye Conjugates Targeting EGFR and HER2 for the Photoimmunotherapy of Bladder Cancer, ANTICANCER RESEARCH 44: 1837-1844 (2024). https://doi.org/10.21873/anticanres.16985

Klemenz, L., Wolf, I., Storz, J., Schultze-Seemann, S., Gratzke, C., Lauw, S., Brückner, R., Wolf, P. (2024). Photoimmunotherapy of HER2-expressing Breast Cancer Cells, CANCER GENOMICS & PROTEOMICS 21: 361-367 (2024). https://doi.org/10.21873/cgp.20453

Hess, S., Alonso, M. C., Brand, M., Lauw, S., Lindenmann, U., Göbel, K.,Geurink, P. P., Fritz, G., Riedl, R., and Knobeloch. K.-P. (2025). A NanoBRET-based assay monitoring interactions in the USP18 signaling hub identifies the first cell-penetrant small molecule compromising USP18/ISG15 binding, bioRxiv preprint. https://doi.org/10.1101/2025.03.30.646166
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