Thank you for your question. We transiently expressed the YFP-ACS5 in tobacco leaves, and we did the immunoprecipitation to get rid of other tobacco proteins. Without IP, you can not check YFP-ACS5 is phosphorylated by MBP-SINAT2 or not, and we used UB-antibody to confirm the ubiquitination of YFP-ACS5. GFP-antibody is already used for IP process, so we can not use GFP-antibody for check the ubiquitination.
2022-07-24 13:12:02