Giada Mattiuzzo National Institute for Biological Standards Control (NIBSC)

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Nigel Temperton
3 protocols

George Carnell Laboratory of Viral Zoonotics, Department of Veterinary Medicine, Cambridge University, Madingley Road, Cambridge
1 protocol

Craig Thompson University of Oxford
1 protocol

Elinor Thompson University of Greenwich
1 protocol

Giada Mattiuzzo

National Institute for Biological Standards and Control (NIBSC)

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3 Author merit
Reviewer merit
Editor merit

3 Protocols published

Production, Titration, Neutralisation, Storage and Lyophilisation of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) Lentiviral Pseudotypes

Authors: Cecilia Di Genova, Alex Sampson, Simon Scott, Diego Cantoni, Martin Mayora-Neto, Emma Bentley, Giada Mattiuzzo, Edward Wright, Mariliza Derveni, Bethany Auld, Bill T. Ferrara, Dale Harrison, Mohamed Said, Arwa Selim, Elinor Thompson, Craig Thompson, George Carnell and Nigel Temperton, date: 11/05/2021, view: 1965, Q&A: 0

This protocol details a rapid and reliable method for the production and titration of high-titre viral pseudotype particles with the SARS-CoV-2 spike protein (and D614G or other variants of concern, VOC) on a lentiviral vector core, and use for

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Optimised Method for the Production and Titration of Lentiviral Vectors Pseudotyped with the SARS-CoV-2 Spike

Authors: Leila Mekkaoui, Emma M. Bentley, Mathieu Ferrari, Katarina Lamb, Katarzyna Ward, Rajeev Karattil, Zulaikha Akbar, Reyisa Bughda, James Sillibourne, Shimobi Onuoha, Giada Mattiuzzo, Yasu Takeuchi and Martin Pule, date: 08/20/2021, view: 1586, Q&A: 0

The use of recombinant lentivirus pseudotyped with the coronavirus Spike protein of SARS-CoV-2 would circumvent the requirement of biosafety-level 3 (BSL-3) containment facilities for the handling of SARS-CoV-2 viruses. Herein, we describe a fast

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