Tianyi Mao
1 protocol

Evan Wilson Oregon Health and Sciences University
1 protocol

Haining Zhong
  • Faculty, Oregon Health & Science University Portland
Research focus
  • two-photon fluorescence lifetime imaging microscopy (2pFLIM); two-photon microscopy; photoactivated localization microscopy (PALM); Endogenous labeling; CRISPR-mediated insertion of exon (CRISPIE); A-kinase activity reporter (AKAR); cAMP Fluorescence Imaging Reporters based on Epac (cAMPFIREs); genetically encoded sensors
  • 1 Author merit

Education

Ph.D., Johns Hopkins University School of Medicine, 2002

Lab information

The Zhong Lab study how the brain change to allow an animal to adapt to the ever-changing world. Our focus is on two types of changes — neuromodulation and experience-dependent plasticity — using rodents as the experimental model. We perform in vitro and in vivo experiments using a variety of approaches, including advanced microscopy, electrophysiology, optogenetics, mouse genetics, CRISPR-based gene editing, and computation.
https://www.hainingzhonglab.com/

Publications

1 Protocol published
Labeling Endogenous Proteins Using CRISPR-mediated Insertion of Exon (CRISPIE)
Authors:  Evan A. Wilson, Tianyi Mao and Haining Zhong, date: 03/05/2022, view: 1876, Q&A: 0

The CRISPR/Cas9 technology has transformed our ability to edit eukaryotic genomes. Despite this breakthrough, it remains challenging to precisely knock-in large DNA sequences, such as those encoding a fluorescent protein, for labeling or modifying a

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