Avnish Patel —BIO-PROTOCOL

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Robert Moon London School of Hygiene and Tropical Medicine, UK
2 protocols

Franziska Mohring London School of Hygiene and Tropical Medicine, UK
2 protocols

David Baker 1 protocol

Melissa Hart 1 protocol

Avnish Patel

1 Author merit
Reviewer merit
Editor merit

1 Protocol published

CRISPR-Cas9 Genome Editing of Plasmodium knowlesi

Authors: Franziska Mohring, Melissa N. Hart, Avnish Patel, David A. Baker and Robert W. Moon, date: 02/20/2020, view: 4392, Q&A: 1

Plasmodium knowlesi is a zoonotic malaria parasite in Southeast Asia that can cause severe and fatal malaria in humans. The main hosts are Macaques, but modern diagnostic tools reveal increasing numbers of human infections. After P. ...

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Annemarie Voorberg-van der Wel

Department of Parasitology, Biomedical Primate Research Centre, 2288 GJ Rijswijk

Dear authors,

Thank you for this excellent protocol. This is very helpful.
Just a note: can you please check the recipe for pyrimethamine? Under 3b the 1:1000 dilution mentioned would result in a 10 uM solution, rather than a 100 uM.

10/20/2020 12:30:26 AM Reply

Annemarie Voorberg-van der Wel

Department of Parasitology, Biomedical Primate Research Centre, 2288 GJ Rijswijk

Great, thank you.

2020-10-22 01:25:09

Franziska Mohring

London School of Hygiene and Tropical Medicine, UK

Thanks for spotting this. It should be 50 ul of the Pyrimethamine aliquot to 5 ml RPMI, instead of 50 ml.
The final concentration in the culture is 100 nM.

2020-10-21 05:53:24