Agnieszka Pastula Umeå University Umeå
6 protocols

Giusy Tornillo Cardiff University
27 protocols

Tomohiro Mizutani
  • Research associate, Tokyo Medical and Dental University
Research focus
  • Stem cell
  • Stem cell biology, Cancer biology, Regenerative medicine


M.D., Ph.D., Tokyo Medical and Dental University, 2012

Lab information

Department of Gastroenterology and Hepatology


1 Protocol reviewed
In vitro Differentiation of Human iPSC-derived Retinal Pigment Epithelium Cells (iPSC-RPE)
Authors:  Agnieszka D’Antonio-Chronowska, Matteo D’Antonio and Kelly A. Frazer, date: 12/20/2019, view: 4436, Q&A: 0
Induced Pluripotent Stem Cells (iPSCs) serve as an excellent model system for studying the molecular underpinnings of tissue development. Human iPSC-derived retinal pigment epithelium (iPSC-RPE) cells have fetal-like molecular profiles. Hence, ...
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2 Protocols edited
Fluidigm Based Single-cell Gene Expression Library Preparation from Patient-derived Small Intestinal Organoids
Authors:  Kohei Suzuki and Ryuichi Okamoto, date: 10/05/2020, view: 1986, Q&A: 0
In this protocol, we describe our methods to isolate crypts from patients' biopsy samples and to culture human intestinal stem cells as it’s called “organoid.” Beyond that, we describe how to dissociate organoids cells into single cells for ...
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In vitro Self-organized Mouse Small Intestinal Epithelial Monolayer Protocol
Authors:  Gizem Altay, Eduard Batlle, Vanesa Fernández-Majada and Elena Martinez, date: 02/05/2020, view: 6180, Q&A: 0
Developing protocols to obtain intestinal epithelial monolayers that recapitulate in vivo physiology to overcome the limitations of the organoids’ closed geometry has become of great interest during the last few years. Most of the developed ...
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