Agnieszka Pastula Uniwerstet Jagiellonski
6 protocols

Giusy Tornillo Cardiff University
49 protocols

Tomohiro Mizutani
  • Research associate, Tokyo Medical and Dental University
Research focus
  • Stem cell biology, Cancer biology, Regenerative medicine


M.D., Ph.D., Tokyo Medical and Dental University, 2012

Lab information

Department of Gastroenterology and Hepatology


1 Protocol reviewed
In vitro Differentiation of Human iPSC-derived Retinal Pigment Epithelium Cells (iPSC-RPE)
Authors:  Agnieszka D’Antonio-Chronowska, Matteo D’Antonio and Kelly A. Frazer, date: 12/20/2019, view: 5803, Q&A: 0
Induced Pluripotent Stem Cells (iPSCs) serve as an excellent model system for studying the molecular underpinnings of tissue development. Human iPSC-derived retinal pigment epithelium (iPSC-RPE) cells have fetal-like molecular profiles. Hence, ...
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3 Protocols edited
Low-viscosity Matrix Suspension Culture for Human Colorectal Epithelial Organoids and Tumoroids
Authors:  Tao Tan, Yumiko Hirokawa, Jordan Clarke, Anuratha Sakthianandeswaren and Oliver M. Sieber, date: 04/20/2022, view: 1644, Q&A: 0

Three-dimensional culture of human normal colorectal epithelium and cancer tissue as organoids and tumoroids has transformed the study of diseases of the large intestine. A widely used strategy for generating patient-derived colorectal organoids and

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Fluidigm Based Single-cell Gene Expression Library Preparation from Patient-derived Small Intestinal Organoids
Authors:  Kohei Suzuki and Ryuichi Okamoto, date: 10/05/2020, view: 2934, Q&A: 0
In this protocol, we describe our methods to isolate crypts from patients' biopsy samples and to culture human intestinal stem cells as it’s called “organoid.” Beyond that, we describe how to dissociate organoids cells into single cells for ...
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