Rezwan Siddiquee University of Sydney Sydney
1 protocol

Jinlong Gao 1 protocol

Anthony Yammine School of Life and Environmental Sciences, The University of Sydney, NSW, 2006, Australia
1 protocol

Ann H Kwan
  • Senior Resaerch Fellow, School of Life and Environmental Sciences, University of Sydney, NSW
Research focus
  • -
  • 2 Author merit


PhD, University of Sydney, Australia, 2004


2 Protocols published
Cell-free Synthesis of Correctly Folded Proteins with Multiple Disulphide Bonds: Production of Fungal Hydrophobins
Authors:  Rezwan Siddiquee and Ann H Kwan, date: 05/20/2021, view: 3256, Q&A: 0

Cell-free synthesis is a powerful technique that uses the transcriptional and translational machinery extracted from cells to create proteins without the constraints of living cells. Here, we report a cell-free protein production protocol using

Tryptophan Fluorescence Quenching Assays for Measuring Protein-ligand Binding Affinities: Principles and a Practical Guide
Authors:  Anthony Yammine, Jinlong Gao and Ann H. Kwan, date: 06/05/2019, view: 15362, Q&A: 0
Tryptophan fluorescence quenching is a type of fluorescence spectroscopy used for binding assays. The assay relies on the ability to quench the intrinsic fluorescence of tryptophan residues within a protein that results from changes in the local ...
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