Further queries as follows:
-In procedure. step no 6. the shaking speed(how much RPM) to induce the expression of the MS2-CP-GFP-SBP is not mentioned. I think the shaking speed is a critical parameter for protein expression.
-For western blot, which primary/secondary antibody should we use to determine MS2-CP-GFP-SBP on the SDS gel. or fluorophore tagged MS2 aptamer can be used to visualize the pull-down fractions?
5/15/2022 12:27:18 PM Reply