Jeffrey E. Gerst Weizmann Institute of Science Rehovot

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Gal Haimovich Weizmann Institute of Science Rehovot
3 protocols

Rohini Nair Weizmann Institute of Science Rehovot
1 protocol

Jeffrey E. Gerst

Faculty, Weizmann Institute of Science Rehovot

Research focus

Intracellular and intercellular RNA trafficking

2 Author merit
Reviewer merit
Editor merit

Education

Ph.D., Weizmann Institute of Science, 1989

Lab information

Jeffrey Gerst Lab
http://www.weizmann.ac.il/molgen/Gerst/

Publications

http://www.weizmann.ac.il/molgen/Gerst/publications

2 Protocols published

An Aptamer-based mRNA Affinity Purification Procedure (RaPID) for the Identification of Associated RNAs (RaPID-seq) and Proteins (RaPID-MS) in Yeast

Authors: Rohini R. Nair, Gal Haimovich and Jeffrey E. Gerst, date: 01/05/2022, view: 1203, Q&A: 2

RNA-RNA and RNA-protein interactions are involved in the regulation of gene expression. Here, we describe an updated and extended version of our RNA purification and protein identification (RaPID) protocol for the pulldown of aptamer-tagged mRNAs by

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ARGHYA SETT

IOCB, Prague

Further queries as follows:
-In procedure. step no 6. the shaking speed(how much RPM) to induce the expression of the MS2-CP-GFP-SBP is not mentioned. I think the shaking speed is a critical parameter for protein expression.
-For western blot, which primary/secondary antibody should we use to determine MS2-CP-GFP-SBP on the SDS gel. or fluorophore tagged MS2 aptamer can be used to visualize the pull-down fractions?

5/15/2022 12:27:18 PM Reply

Rohini Nair

Weizmann Institute of Science

-In procedure. step no 6. the shaking speed(how much RPM) to induce the expression of the MS2-CP-GFP-SBP is not mentioned. I think the shaking speed is a critical parameter for protein expression.
170rpm
-For western blot, which primary/secondary antibody should we use to determine MS2-CP-GFP-SBP on the SDS gel. or fluorophore tagged MS2 aptamer can be used to visualize the pull-down fractions?
anti-GFP

2022-05-19 18:03:00

Rohini Nair

Weizmann Institute of Science

2022-05-19 18:02:59

Single-molecule Fluorescence in situ Hybridization (smFISH) for RNA Detection in Adherent Animal Cells

Authors: Gal Haimovich and Jeffrey E. Gerst, date: 11/05/2018, view: 27494, Q&A: 2

Transcription and RNA decay play critical roles in the process of gene expression and the ability to accurately measure cellular mRNA levels is essential for understanding this regulation. Here, we describe a single-molecule fluorescent in situ ...

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