Douglas Gladue Agricultural Research Service Plum Island Animal Disease Center, Greenport, USA, USA,
2 protocols

Manuel Borca USDA-ARS Orient Point
2 protocols

Consuelo Carrillo USDA-APHIS Orient Point
1 protocol

Lauro Velazquez-Salinas USDA-ARS Orient Point
1 protocol

Elizabeth Ramirez-Medina
  • Research scientist, USDA-ARS Orient Point
  • 2 Author merit


Autonomous Metropolitan University


2 Protocols published
Detection and Quantification of African Swine Fever Virus in MA-104 Cells

Detection of live African swine fever virus (ASFV) has historically relied on the use of primary swine macrophages (PSM). PSM do not replicate and have to be isolated fresh from donor swine. We previously identified that a MA-104 cells (ATCC

CRISPR/Cas Gene Editing of a Large DNA Virus: African Swine Fever Virus
Gene editing of large DNA viruses, such as African swine fever virus (ASFV), has traditionally relied on homologous recombination of a donor plasmid consisting of a reporter cassette with surrounding homologous viral DNA. However, this homologous ...
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