Dear Dr.Wang, When I transformed the TevCas9 vector into the Phaeodactylum tricornutum, I found there were multiple cuts of the target gene of the single clone grown on the zeocin plate . I only design the gRNA of Cas9 and ignore the TEV site. I used gene gunning to bombard the vector into the Phaeodactylum tricornutum. I would appreciate it if you could help me solve this problem. My Emai is Liuxuehua1213@163.com 9/18/2019 10:07:58 AM Reply