Andrea Puhar MIMS and Department of Molecular Biology, Umeå University
53 protocols

Chiara Ambrogio University of Turin
48 protocols

John Peterson Cleveland Clinic Foundation
3 protocols

Sumeet Nayak University of Massachusetts Medical School
2 protocols

Editor
Valerian DORMOY
  • Faculty, Inserm
Research focus
  • Cell biology
  • Respiratory diseases, histology, ciliogenesis

Education

PhD, University of Strasbourg, 2010

Publications

6 Protocols reviewed
Isolation of Myofibres and Culture of Muscle Stem Cells from Adult Zebrafish
Authors:  Massimo Ganassi, Peter S. Zammit and Simon M. Hughes, date: 09/05/2021, view: 1412, Q&A: 0

Skeletal muscles generate force throughout life and require maintenance and repair to ensure efficiency. The population of resident muscle stem cells (MuSCs), termed satellite cells, dwells beneath the basal lamina of adult myofibres and contributes

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Dual Fluorescence Cytometry Assay to Assess Cellular Protein Levels
Authors:  Ashley M Ngo, Ruofan Wang and Andreas S Puschnik, date: 04/20/2020, view: 2830, Q&A: 0
Expression levels of cellular proteins can be affected by various perturbations, such as genetic knockout of interactors, drug treatments or cell stress. To specifically measure the effects on protein levels post-synthesis under different ...
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1 Protocol edited
Extraction of DNA and RNA from Formalin-fixed Paraffin-embedded Tissue Specimens

One of the most common methods for isolating biomolecules from clinical samples is using TRIzol. While many tissues are freshly frozen, a large number of tissues are formalin-fixed paraffin-embedded (FFPE) blocks for pathology and diagnostics.

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