Douglas Paul Gladue Agricultural Research Service Plum Island Animal Disease Center, Greenport, USA,

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Manuel Borca USDA-ARS Orient Point
2 protocols

Consuelo Carrillo USDA-APHIS Orient Point
1 protocol

Lauro Velazquez-Salinas USDA-ARS Orient Point
1 protocol

Ediane Silva USDA-ARS Orient Point
1 protocol

Douglas Paul Gladue

Agricultural Research Service Plum Island Animal Disease Center, Greenport, USA, USA,

Research focus

Microbiology

2 Author merit
Reviewer merit
Editor merit

Education

Ph.D, Stony Brook University, USA, 2007

Current position

Senior Research Scientist, Plum Island Animal Disease Center, Agricultural Research Service, USDA, USA

Publications

https://scholar.google.com/citations?user=csyqsS0AAAAJ&hl=en

2 Protocols published

Detection and Quantification of African Swine Fever Virus in MA-104 Cells

Authors: Ayushi Rai, Sarah Pruitt, Elizabeth Ramirez-Medina, Elizabeth A. Vuono, Ediane Silva, Lauro Velazquez-Salinas, Consuelo Carrillo, Manuel V. Borca and Douglas P. Gladue, date: 03/20/2021, view: 2794, Q&A: 0

Detection of live African swine fever virus (ASFV) has historically relied on the use of primary swine macrophages (PSM). PSM do not replicate and have to be isolated fresh from donor swine. We previously identified that a MA-104 cells (ATCC

...

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CRISPR/Cas Gene Editing of a Large DNA Virus: African Swine Fever Virus

Authors: Manuel V. Borca, Keith A. Berggren, Elizabeth Ramirez-Medina, Elizabeth A. Vuono and Douglas P. Gladue, date: 08/20/2018, view: 7436, Q&A: 0

Gene editing of large DNA viruses, such as African swine fever virus (ASFV), has traditionally relied on homologous recombination of a donor plasmid consisting of a reporter cassette with surrounding homologous viral DNA. However, this homologous ...

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