Chaoxi Luo —BIO-PROTOCOL

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Weixiao Yin Huazhong Agricultural University
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Chaoxi Luo

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Plant science

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1 Protocol published

Functional Evaluation of the Signal Peptides of Secreted Proteins

Authors: Weixiao Yin, Yufu Wang, Tao Chen, Yang Lin and Chaoxi Luo, date: 05/05/2018, view: 9107, Q&A: 4

Here, we describe a method that can be used to evaluate the function of predicted signal peptides. This method utilizes the yeast Saccharomyces cerevisiae YTK12 strain and pSUC2 vector in which the pSUC2 vector with fused predicted signal ...

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Emilee Storfie

University of Alberta

For the colour observation step, was there a certain OD600 that should be obtained before harvesting the cells? And did you confirm your YTK12 transformants via PCR or just by growth on the different medium? Thanks!

11/24/2021 10:25:05 PM Reply

Emilee Storfie

University of Alberta

Awesome, thanks!

2022-01-03 14:19:27

Weixiao Yin

Huazhong Agricultural University

1. In our assay, we did not measure OD600, and I think it is a good idea. Because it is possible to observe different degree level if red when you test different signal peptides.
2. For the first, we select the colonies that can grow on the minus Trp medium, and then the colonies will be confirmed via PCR. The positive colonies are selected for next steps.

2021-12-01 16:40:36