Jens Gruber Medical RNA Biology, German Primate Center, Germany, Germany,
1 protocol

Liezel Tamon Medical RNA Biology, German Primate Center, Germany, Germany,
1 protocol

Nicolas Lemus-Diaz
  • Post-Doc, Molecular Biology,UMG, Goettingen Germany
Research focus
  • Molecular biology
  • RNA biology
    miRNA, snoRNA and snRNA
    Gene delivery tools
    iRNA as gene therapy
  • 1 Author merit


MD - PhD, Universidad Nacional de Colombia (MD) and Georg-August-Universität Göttingen - IMPRS for Molecular Biology (PhD)

Lab information

AG Bohnsack


1. Schiller LT, Lemus-Diaz N, Rinaldi Ferreira R, Böker KO, Gruber J. Enhanced
Production of Exosome-Associated AAV by Overexpression of the Tetraspanin CD9.
Mol Ther Methods Clin Dev. 2018 Mar 29;9:278-287. doi:
10.1016/j.omtm.2018.03.008. eCollection 2018 Jun 15. PubMed [citation] PMID:29707602, PMCID: PMC5918177

2. Böker KO, Lemus-Diaz N, Rinaldi Ferreira R, Schiller L, Schneider S, Gruber J.
The Impact of the CD9 Tetraspanin on Lentivirus Infectivity and Exosome
Secretion. Mol Ther. 2018 Feb 7;26(2):634-647. doi:10.1016/j.ymthe.2017.11.008.
Epub 2017 Nov 16. PubMed [citation] PMID: 29221804, PMCID: PMC5835022

3. Lemus-Diaz N, Böker KO, Rodriguez-Polo I, Mitter M, Preis J, Arlt M, Gruber J.
Dissecting miRNA gene repression on single cell level with an advanced
fluorescent reporter system. Sci Rep. 2017 Mar 24;7:45197. doi:
10.1038/srep45197. PubMed [citation] PMID: 28338079, PMCID: PMC5364550

4. Effects of RANKL Knockdown by Virus-like Particle-Mediated RNAi in a Rat Model of
Hoffmann DB, Gruber J, Böker KO, Deppe D, Sehmisch S, Schilling AF, Lemus-Diaz N,
Komrakova M, Schneider S.
Molecular Therapy. Nucleic Acids. 2018 Jul 11; 12: 443-452
PMC [article] PMCID: PMC6041464, PMID: 0, DOI:10.1016/j.omtn.2018.06.001
1 Protocol published
Dual Fluorescence Reporter Based Analytical Flow Cytometry for miRNA Induced Regulation in Mammalian Cells
Authors:  Nicolas Lemus-Diaz, Liezel Tamon and Jens Gruber, date: 09/05/2018, view: 9632, Q&A: 0
MicroRNA-induced gene regulation is a growing field in basic and translational research. Examining this regulation directly in cells is necessary to validate high-throughput data originated from RNA sequencing technologies. For this several studies ...
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