Ariane Renaud —BIO-PROTOCOL

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Sylvain Moineau Université Laval
1 protocol

Geneviève Rousseau 1 protocol

Marie-Laurence Lemay 1 protocol

Ariane Renaud

Research focus

Microbiology

1 Author merit
Reviewer merit
Editor merit

Education

B.Sc. in Microbiology, Université Laval, Canada, 2016

Current position

Master Student, Département de biochimie, microbiologie et bio-informatique, Université Laval, Canada

Publications

None yet !

1 Protocol published

Targeted Genome Editing of Virulent Phages Using CRISPR-Cas9

Authors: Marie-Laurence Lemay, Ariane C. Renaud, Geneviève M. Rousseau and Sylvain Moineau, date: 01/05/2018, view: 14592, Q&A: 1

This protocol describes a straightforward method to generate specific mutations in the genome of strictly lytic phages. Briefly, a targeting CRISPR-Cas9 system and a repair template suited for homologous recombination are provided inside a bacterial ...

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Alex Sem

Phagelux Canada

I work with another phages and another host bacteria (Staphylococcus, Klebsiella) and need to modify this protocol a lot.

12/18/2018 12:02:27 PM Reply

Sylvain Moineau

Université Laval

Thanks for your comments. Indeed the protocol needs to be adapted to other hosts. Others have been very successful in adapting it to E. coli. Pseudomonas, etc. So I am not sure what are the issues. Please do not hesitate to let us know !

2018-12-18 13:06:21

Marie-Laurence Lemay

Hi Alex,
Thanks for your comment. Could you share the most significant changes? This might help other readers.

2018-12-18 12:29:02