M. Anleu Gil Department of Biology, Stanford University, USA, USA,
1 protocol

Nidhi Sharma HHMI/Stanford University
2 protocols

Diego Wengier
Research focus
  • Microbiology
  • 1 Author merit


Doctor, Universidad de Buenos Aires, Argentina, 2008

Current position

Assistant Investigator, Instituto de Investigaciones en Ingenieria Genetica y Biologia Molecular "Dr. Hector Torres", Consejo Nacional de Investigaciones Cientificas y Tecnicas, Argentina


  1. Muschietti, J. P. and Wengier, D. L. (2018). How many receptor-like kinases are required to operate a pollen tube. Current Opinions in Plant Biology 41: 73-82.
  2. Wengier, D. L., Lampard, G. R. and Bergmann, D. C. (2017). Dissection of MAPK signaling specificity through protein engineering in a developmental context. BioRxiv.
  3. Lampard, G. R., Wengier, D. L. and Bergmann, D. C. (2014). Manipulation of mitogen-activated protein kinase kinase signaling in the Arabidopsis stomatal lineage reveals motifs that contribute to protein localization and signaling specificity. Plant Cell 26(8): 3358-3371.
  4. Wengier, D. L. and Bergmann, D. C. (2012). On fate and flexibility in stomatal development. Cold Spring Harb Symp Quant Biol 77: 53-62.
  5. Salem, T. M., Barberini, M. L., Wengier, D. L., Cabanas, M. L., de Paz, P. and Muschietti, J. (2012). Oligomerization studies show that the kinase domain of the tomato pollen receptor kinase LePRK2 is necessary for interaction with LePRK1. Plant Physiol Biochem 53: 40-45.
  6. Salem, T., Mazzella, A., Barberini, M. L., Wengier, D., Motillo, V., Parisi, G. and Muschietti, J. (2011). Mutations in two putative phosphorylation motifs in the tomato pollen receptor kinase LePRK2 show antagonistic effects on pollen tube length. J Biol Chem 286(6): 4882-4891.
  7. Wengier, D. L., Mazzella, M. A., Salem, T. M., McCormick, S. and Muschietti, J. P. (2010). STIL, a peculiar molecule from styles, specifically dephosphorylates the pollen receptor kinase LePRK2 and stimulates pollen tube growth in vitro. BMC Plant Biol 10: 33.
  8. Zhang, D., Wengier, D., Shuai, B., Gui, C. P., Muschietti, J., McCormick, S. and Tang, W. H. (2008). The pollen receptor kinase LePRK2 mediates growth-promoting signals and positively regulates pollen germination and tube growth. Plant Physiol 148(3): 1368-1379.
  9. Blaustein, M., Pelisch, F., Tanos, T., Munoz, M. J., Wengier, D., Quadrana, L., Sanford, J. R., Muschietti, J. P., Kornblihtt, A. R., Caceres, J. F., Coso, O. A. and Srebrow, A. (2005). Concerted regulation of nuclear and cytoplasmic activities of SR proteins by AKT. Nat Struct Mol Biol 12(12): 1037-1044.
  10. Kaothien, P., Ok, S. H., Shuai, B., Wengier, D., Cotter, R., Kelley, D., Kiriakopolos, S., Muschietti, J. and McCormick, S. (2005). Kinase partner protein interacts with the LePRK1 and LePRK2 receptor kinases and plays a role in polarized pollen tube growth. Plant J 42(4): 492-503.
  11. Wengier, D., Valsecchi, I., Cabanas, M. L., Tang, W. H., McCormick, S. and Muschietti, J. (2003). The receptor kinases LePRK1 and LePRK2 associate in pollen and when expressed in yeast, but dissociate in the presence of style extract. Proc Natl Acad Sci U S A 100(11): 6860-6865.
1 Protocol published
A Quick and Easy Method for Making Competent Escherichia coli Cells for Transformation Using Rubidium Chloride
Authors:  Nidhi Sharma, M. Ximena Anleu Gil and Diego Wengier, date: 11/05/2017, view: 17877, Q&A: 2
This protocol describes a quick and efficient method to make competent E. coli cells for transformation using rubidium chloride. Commercial competent cells are expensive and this protocol provides a cheaper alternative to them.
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