Emanuela Gussoni Boston Children's Hospital
1 protocol

Janelle M Spinazzola
Research focus
  • Stem cell
  • 1 Author merit


PhD, University of Pennsylvania, USA, 2015

Current position

Research Fellow, Genetics and Genomics, Boston Children's Hospital, USA


  1. Vieira, N. M., Spinazzola, J. M., Alexander, M. S., Moreira, Y. B., Kawahara, G., Gibbs, D. E., Mead, L. C., Verjovski-Almeida, S., Zatz, M. and Kunkel, L. M. (2017). Repression of phosphatidylinositol transfer protein alpha ameliorates the pathology of Duchenne muscular dystrophy. Proc Natl Acad Sci U S A 114(23): 6080-6085.
  2. Spinazzola, J. M. and Gussoni, E. (2017). Exosomal Small Talk Carries Strong Messages from Muscle Stem Cells. Cell Stem Cell 20(1): 1-3.
  3. Spinazzola, J. M. and Kunkel, L. M. (2016). Pharmacological therapeutics targeting the secondary defects and downstream pathology of Duchenne muscular dystrophy. Expert Opin Orphan Drugs 4(11): 1179-1194.
  4. Alexander, M. S., Rozkalne, A., Colletta, A., Spinazzola, J. M., Johnson, S., Rahimov, F., Meng, H., Lawlor, M. W., Estrella, E., Kunkel, L. M. and Gussoni, E. (2016). CD82 Is a Marker for Prospective Isolation of Human Muscle Satellite Cells and Is Linked to Muscular Dystrophies. Cell Stem Cell 19(6): 800-807.
  5. Alexander, M. S., Gasperini, M. J., Tsai, P. T., Gibbs, D. E., Spinazzola, J. M., Marshall, J. L., Feyder, M. J., Pletcher, M. T., Chekler, E. L., Morris, C. A., Sahin, M., Harms, J. F., Schmidt, C. J., Kleiman, R. J. and Kunkel, L. M. (2016). Reversal of neurobehavioral social deficits in dystrophic mice using inhibitors of phosphodiesterases PDE5A and PDE9A. Transl Psychiatry 6(9): e901.
  6. Philippou, A., Minozzo, F. C., Spinazzola, J. M., Smith, L. R., Lei, H., Rassier, D. E. and Barton, E. R. (2015). Masticatory muscles of mouse do not undergo atrophy in space. FASEB J 29(7): 2769-2779.
  7. Spinazzola, J. M., Smith, T. C., Liu, M., Luna, E. J. and Barton, E. R. (2015). Gamma-sarcoglycan is required for the response of archvillin to mechanical stimulation in skeletal muscle. Hum Mol Genet 24(9): 2470-2481.
  8. Moorwood, C., Philippou, A., Spinazzola, J., Keyser, B., Macarak, E. J. and Barton, E. R. (2014). Absence of gamma-sarcoglycan alters the response of p70S6 kinase to mechanical perturbation in murine skeletal muscle. Skelet Muscle 4: 13.
  9. Brisson, B. K., Spinazzola, J., Park, S. and Barton, E. R. (2014). Viral expression of insulin-like growth factor I E-peptides increases skeletal muscle mass but at the expense of strength. Am J Physiol Endocrinol Metab 306(8): E965-974.
  10. Park, S., Brisson, B. K., Liu, M., Spinazzola, J. M. and Barton, E. R. (2014). Mature IGF-I excels in promoting functional muscle recovery from disuse atrophy compared with pro-IGF-IA. J Appl Physiol (1985) 116(7): 797-806.
  11. Forbes, S. C., Bish, L. T., Ye, F., Spinazzola, J., Baligand, C., Plant, D., Vandenborne, K., Barton, E. R., Sweeney, H. L. and Walter, G. A. (2014). Gene transfer of arginine kinase to skeletal muscle using adeno-associated virus. Gene Ther 21(4): 387-392.
  12. Vandenburgh, H., Shansky, J., Benesch-Lee, F., Skelly, K., Spinazzola, J. M., Saponjian, Y. and Tseng, B. S. (2009). Automated drug screening with contractile muscle tissue engineered from dystrophic myoblasts. FASEB J 23(10): 3325-3334.
1 Protocol published
Isolation of Primary Human Skeletal Muscle Cells
Authors:  Janelle M. Spinazzola and Emanuela Gussoni, date: 11/05/2017, view: 7913, Q&A: 0
Primary myoblast culture is a valuable tool in research of muscle disease, pathophysiology, and pharmacology. This protocol describes techniques for dissociation of cells from human skeletal muscle biopsies and enrichment for a highly myogenic ...
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