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Carlos Duarte
CNC-Center for Neuroscience and Cell Biology, University of Coimbra, Portugal, Portugal,
2 protocols
Simone Frisari
Scuola Internazionale Studi Superiori Avanzati (SISSA), Trieste
1 protocol
Rui Costa
CNC-Center for Neuroscience and Cell Biology, University of Coimbra, Portugal, Portugal,
1 protocol
Margarida Caldeira
CNC-Center for Neuroscience and Cell Biology, University of Coimbra, Portugal
2 protocols
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Bárbara Correia
CNC-Center for Neuroscience and Cell Biology, University of Coimbra, Portugal, Portugal,
Research focus
Neuroscience
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1
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1 Protocol published
Preparation of Primary Cultures of Embryonic Rat Hippocampal and Cerebrocortical Neurons
Authors:
Ivan L. Salazar
,
Miranda Mele
,
Margarida V. Caldeira
,
Rui O. Costa
,
Bárbara Correia
,
Simone Frisari
and
Carlos B. Duarte
,
date:
09/20/2017,
view:
15447,
Q&A:
0
This protocol aims at standardizing the procedure to obtain primary cultures of hippocampal and cerebrocortical neurons for
in vitro
experiments. Cultures should be prepared from cells isolated during embryonic development when neuronal ...
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Tingting chen
18275112414
7/8/2018 3:46:04 PM
Reply
豪 文
上海药明康德新药开发有限公司
when you blowing the tissue to single cell, yo may force this too harsh. Maybe you can change the medium after 2 hours adhere, slightly shaking the dish before change the medium.
2021-04-01 12:41:14
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