Akira Karasawa
  • Staff scientist, New York Structural Biology Center
Research fields
  • Biochemistry, Biophysics
Personal information


PhD in Biology, Osaka University, 2008

Lab information

Research focus

Membrane protein biochemistry/biophysics

1. Karasawa, A. and Kawate, T. High-throughput YO-PRO-1 Uptake Assay for P2X7 Receptors Expressed in HEK Cells.
Bio-101: e2943 (2018)

2. Karasawa, A., Michalski, K., Mikhelzon, P., and Kawate, T.
The P2X7 receptor forms a dye-permeable pore independent of its intracellular domain but dependent on membrane lipid composition.
Elife 6, e31186 (2017)

3. Karasawa, A., and Kawate, T.
Expression and Purification of a Mammalian P2X7 Receptor from Sf9 Insect Cells.
Bio-protocol 7, e2544 (2017).

4. Karasawa, A., and Kawate, T.
Structural basis for subtype-specific inhibition of the P2X7 receptor.
Elife 5, e22153 (2016)

5. Karasawa A., Swier L.J., Stuart M.C., Brouwers J., Helms B., Poolman B.
Physicochemical factors controlling the activity and energy coupling of an ionic strength-gated ATP-binding cassette (ABC) transporter.
J. Biol. Chem. 288, 29862-71 (2013)

6. Gul N., Schuurman-Wolters G., Karasawa A., Poolman B.
Functional characterization of amphipathic a-helix in the osmoregulatory ABC transporter OpuA.
Biochemistry. 51, 5142-52 (2012)

7. Karasawa A., Erkens G.B., Berntsson R.P., Otten R., Schuurman-Wolters G.K., Mulder F.A., Poolman B.
Cystathionine b synthase CBS domains 1 and 2 fulfill different roles in ionic strength-sensing of the ABC transporter OpuA.
J. Biol. Chem. 286, 37280-37291, (2011)

8. Wolters J.C., Berntsson R.P., Gul N., Karasawa A, Thunnissen A.M., Slotboom D.J., Poolman B.
Ligand binding and crystal structures of the substrate-binding domain of the ABC transporter OpuA.
PLoS One. 5: e10361 (2010)

9. Karasawa A, Mitsui K., Matsushita M., Kanazawa H.
Intermolecular cross-linking of monomers in H. pylori Na+/H+ antiporter NhaA at dimer interface inhibits antiporter activity.
Biochem J. 426, 99–108 (2010)

10. Karasawa A., Mitsui K., Matsushita M., Kanazawa H.
Functional Assembly of the Na+/H+ Antiporter of Helicobacter pylori from Partial Fragments in vivo.
Biochemistry. 46, 14272-83 (2007)

11. Karasawa A., Tsuboi Y., Inoue H., Kinoshita R., Nakamura N., Kanazawa H.
Detection of oligomerization and conformational changes in the Na+/H+ antiporter from Helicobacter pylori by fluorescence resonance energy transfer.
J. Biol. Chem. 280, 41900-41911, (2005)
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