Education
Ph.D, University of Tartu, Estonia (defence in Institute of Microbiology, Minsk, Bielorussia), 1986
Current position
Associated Professor , Genetics, Institute of Molecular and Cell Biology, University of Tartu, Estonia
Publications (since 1994)
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Mardo, K., Visnapuu, T., Vija, H., Aasamets, A., Viigand, K. and Alamae, T. (2017). A Highly Active Endo-Levanase BT1760 of a Dominant Mammalian Gut Commensal Bacteroides thetaiotaomicron Cleaves Not Only Various Bacterial Levans, but Also Levan of Timothy Grass. PLoS One 12(1): e0169989.
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Bondarenko, O. M., Ivask, A., Kahru, A., Vija, H., Titma, T., Visnapuu, M., Joost, U., Pudova, K., Adamberg, S., Visnapuu, T. and Alamae, T. (2016). Bacterial polysaccharide levan as stabilizing, non-toxic and functional coating material for microelement-nanoparticles. Carbohydr Polym 136: 710-720.
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Viigand, K., Visnapuu, T., Mardo, K., Aasamets, A. and Alamae, T. (2016). Maltase protein of Ogataea (Hansenula) polymorpha is a counterpart to the resurrected ancestor protein ancMALS of yeast maltases and isomaltases. Yeast 33(8): 415-432.
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Adamberg, K., Tomson, K., Talve, T., Pudova, K., Puurand, M., Visnapuu, T., Alamae, T. and Adamberg, S. (2015). Levan Enhances Associated Growth of Bacteroides, Escherichia, Streptococcus and Faecalibacterium in Fecal Microbiota. PLoS One 10(12): e0144042.
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Visnapuu, T., Mardo, K. and Alamae, T. (2015). Levansucrases of a Pseudomonas syringae pathovar as catalysts for the synthesis of potentially prebiotic oligo- and polysaccharides. N Biotechnol 32(6): 597-605.
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Adamberg, S., Tomson, K., Vija, H., Puurand, M., Kabanova, N., Visnapuu, T., Jogi, E., Alamae, T. and Adamberg, K. (2014). Degradation of Fructans and Production of Propionic Acid by Bacteroides thetaiotaomicron are Enhanced by the Shortage of Amino Acids. Front Nutr 1: 21.
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Mardo, K., Visnapuu, T., Gromkova, M., Aasamets, A., Viigand, K., Vija, H. and Alamae, T. (2014). High-throughput assay of levansucrase variants in search of feasible catalysts for the synthesis of fructooligosaccharides and levan. Molecules 19(6): 8434-8455.
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Mardo, K., Visnapuu, T., Vija, H., Elmi, T. and Alamae, T. (2014). Mutational analysis of conserved regions harboring catalytic triad residues of the levansucrase protein encoded by the lsc-3 gene (lsc3) of Pseudomonas syringae pv. tomato DC3000. Biotechnol Appl Biochem 61(1): 11-22.
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Adamberg, S., Tomson, K., Vija, H., Puurand, M., Kabanova, N., Visnapuu, T., Jogi, E., Alamae, T. and Adamberg, K. (2014). Degradation of Fructans and Production of Propionic Acid by Bacteroides thetaiotaomicron are Enhanced by the Shortage of Amino Acids. Front Nutr 1: 21.
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Suppi, S., Michelson, T., Viigand, K. and Alamae, T. (2013). Repression vs. activation of MOX, FMD, MPP1 and MAL1 promoters by sugars in Hansenula polymorpha: the outcome depends on cell's ability to phosphorylate sugar. FEMS Yeast Res 13(2): 219-232.
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Pollumaa, L., Alamae, T. and Mae, A. (2012). Quorum sensing and expression of virulence in pectobacteria. Sensors (Basel) 12(3): 3327-3349.
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Alamäe, T., Visnapuu, T., Mardo, K., Mäe, A. Zamfir, A. D. (2012). Levansucrases of Pseudomonas bacteria: novel approaches for protein expression, assay of enzymes, fructooligosaccharides and heterooligofructans. In: Rauter, A. P. and Lindhorst, T. K. (Eds). Carbohydrate Chemistry. Cambridge, UK: Royal Soc Chemistry, 176–191.
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Visnapuu, T., Mardo, K., Mosoarca, C., Zamfir, A. D., Vigants, A. and Alamae, T. (2011). Levansucrases from Pseudomonas syringae pv. tomato and P. chlororaphis subsp. aurantiaca: substrate specificity, polymerizing properties and usage of different acceptors for fructosylation. J Biotechnol 155(3): 338-349.
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Visnapuu, T., Mäe, A. and Alamäe, T. (2008). Hansenula polymorpha maltase gene promoter with sigma 70-like elements is feasible for Escherichia coli-based biotechnological applications: expression of three genomic levansucrase genes of Pseudomonas syringae pv. tomato. Process Biochem 43(4): 414-422.
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Viigand, K. and Alamae, T. (2007). Further study of the Hansenula polymorpha MAL locus: characterization of the alpha-glucoside permease encoded by the HpMAL2 gene. FEMS Yeast Res 7(7): 1134-1144.
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Andresen, L., Koiv, V., Alamae, T. and Mae, A. (2007). The Rcs phosphorelay modulates the expression of plant cell wall degrading enzymes and virulence in Pectobacterium carotovorum ssp. carotovorum. FEMS Microbiol Lett 273(2): 229-238.
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Viigand, K., Tammus, K. and Alamae, T. (2005). Clustering of MAL genes in Hansenula polymorpha: cloning of the maltose permease gene and expression from the divergent intergenic region between the maltose permease and maltase genes. FEMS Yeast Res 5(11): 1019-1028.
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Karp, H., Jarviste, A., Kriegel, T. M. and Alamae, T. (2003). Cloning and biochemical characterization of hexokinase from the methylotrophic yeast Hansenula polymorpha. Curr Genet 44(5): 268-276.
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Alamae, T., Parn, P., Viigand, K. and Karp, H. (2003). Regulation of the Hansenula polymorpha maltase gene promoter in H. polymorpha and Saccharomyces cerevisiae1. FEMS Yeast Res 4(2): 165-173.
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Laht, S., Karp, H., Kotka, P., Jarviste, A. and Alamae, T. (2002). Cloning and characterization of glucokinase from a methylotrophic yeast Hansenula polymorpha: different effects on glucose repression in H. polymorpha and Saccharomyces cerevisiae. Gene 296(1-2): 195-203.
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Liiv, L., Parn, P. and Alamae, T. (2001). Cloning of maltase gene from a methylotrophic yeast, Hansenula polymorpha. Gene 265(1-2): 77-85.
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Alamae, T. and Jarviste, A. (1995). Permeabilization of the methylotrophic yeast pichia-pinus for intracellular enzyme analysis - a quantitative study. J Microbiol Meth 22(2): 193-205.
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Alamae, T. and Simisker, J. (1994). Isolation and preliminary characterization of Pichia pinus mutants insensitive to glucose repression. Yeast 10(11): 1459-1466.