Hi Mahesh,
I have not worked with rice leaves before. Did you try the 7:1 solution of 95% ethanol: acetic acid as mentioned in this protocol? You can try clearing for a longer time e.g., 2 days, and change the clearing solution twice a day in case the flag leaf is big and thick. Let me know if that works!
12/20/2021 11:37:40 AM Reply