Tino Krell Department of Environmental Protection, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, Spain
1 protocol

Andrés Corral-Lugo Department of Environmental Protection, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, Spain
1 protocol

Míriam Rico Department of Environmental Protection, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, Spain
1 protocol

Vanina García The University of Nottingham
1 protocol
David Paul MRC Laboratory of Molecular Biology
42 protocols

David Cisneros Umeå University
33 protocols

Emilia Krypotou Yale University
20 protocols

Emilie Besnard BioAge Labs
10 protocols

Reviewer
Jose Antonio Reyes-Darias
  • Vircell S.L.
Research focus
  • Microbiology
  • 1 Author merit

Education

Ph.D. in Biochemistry and Molecular Biology, Department of Genetics, University of Georgia, USA, 2012

Current position

Laboratory head of the department of Molecular Biology in the biotechnology company Vircell Spain (Granada, Spain), specialized in the development and production of diagnostic reagents for human infectious diseases.

Publications (since 2008)

  1. Fernandez, M., Morel, B., Corral-Lugo, A., Rico-Jimenez, M., Martin-Mora, D., Lopez-Farfan, D., Reyes-Darias, J. A., Matilla, M. A., Ortega, A. and Krell, T. (2016). Identification of ligands for bacterial sensor proteins. Curr Genet 62(1): 143-147.
  2. Martin-Mora, D., Reyes-Darias, J. A., Ortega, A., Corral-Lugo, A., Matilla, M. A. and Krell, T. (2015). McpQ is a specific citrate chemoreceptor that responds preferentially to citrate/metal ion complexes. Environ Microbiol. (Epub ahead of print)
  3. Garcia, V., Reyes-Darias, J. A., Martin-Mora, D., Morel, B., Matilla, M. A. and Krell, T. (2015). Identification of a Chemoreceptor for C2 and C3 Carboxylic Acids. Appl Environ Microbiol 81(16): 5449-5457.
  4. Reyes-Darias, J. A., Garcia, V., Rico-Jimenez, M., Corral-Lugo, A., Lesouhaitier, O., Juarez-Hernandez, D., Yang, Y., Bi, S., Feuilloley, M., Munoz-Rojas, J., Sourjik, V. and Krell, T. (2015). Specific gamma-aminobutyrate chemotaxis in pseudomonads with different lifestyle. Mol Microbiol 97(3): 488-501.
  5. Reyes-Darias, J. A., Sanchez-Luque, F. J., Morales, J. C., Perez-Rentero, S., Eritja, R. and Berzal-Herranz, A. (2015). Glucose conjugation of anti-HIV-1 oligonucleotides containing unmethylated CpG motifs reduces their immunostimulatory activity. Chembiochem 16(4): 584-591.
  6. Reyes-Darias, J. A., Yang, Y., Sourjik, V. and Krell, T. (2015). Correlation between signal input and output in PctA and PctB amino acid chemoreceptor of Pseudomonas aeruginosa. Mol Microbiol 96(3): 513-525. (Epub ahead of print)
  7. Mayola, A., Irazoki, O., Martinez, I. A., Petrov, D., Menolascina, F., Stocker, R., Reyes-Darias, J. A., Krell, T., Barbe, J. and Campoy, S. (2014). RecA protein plays a role in the chemotactic response and chemoreceptor clustering of Salmonella enterica. PLoS One 9(8): e105578.
  8. Reyes-Darias, J. A. and Berzal-Herranz, A. (2014). Detection of immune response activation by exogenous nucleic acids by a multiplex RT-PCR method. Mol Cell Probes 28(4): 181-185.
  9. Garcia-Fontana, C., Reyes-Darias, J. A., Munoz-Martinez, F., Alfonso, C., Morel, B., Ramos, J. L. and Krell, T. (2013). High specificity in CheR methyltransferase function: CheR2 of Pseudomonas putida is essential for chemotaxis, whereas CheR1 is involved in biofilm formation. J Biol Chem 288(26): 18987-18999.
  10. Lacal, J., Reyes-Darias, J. A., Garcia-Fontana, C., Ramos, J. L. and Krell, T. (2013). Tactic responses to pollutants and their potential to increase biodegradation efficiency. J Appl Microbiol 114(4): 923-933.
  11. Pineda-Molina, E., Reyes-Darias, J. A., Lacal, J., Ramos, J. L., Garcia-Ruiz, J. M., Gavira, J. A. and Krell, T. (2012). Evidence for chemoreceptors with bimodular ligand-binding regions harboring two signal-binding sites. Proc Natl Acad Sci U S A 109(46): 18926-18931.
  12. Krell, T., Lacal, J., Reyes-Darias, J. A., Jimenez-Sanchez, C., Sungthong, R. and Ortega-Calvo, J. J. (2013). Bioavailability of pollutants and chemotaxis. Curr Opin Biotechnol 24(3): 451-456.
  13. Reyes-Darias, J. A., Sanchez-Luque, F. J. and Berzal-Herranz, A. (2012). HIV RNA dimerisation interference by antisense oligonucleotides targeted to the 5' UTR structural elements. Virus Res 169(1): 63-71.
  14. Krell, T., Lacal, J., Guazzaroni, M. E., Busch, A., Silva-Jimenez, H., Fillet, S., Reyes-Darias, J. A., Munoz-Martinez, F., Rico-Jimenez, M., Garcia-Fontana, C., Duque, E., Segura, A. and Ramos, J. L. (2012). Responses of Pseudomonas putida to toxic aromatic carbon sources. J Biotechnol 160(1-2): 25-32.
  15. Lacal, J., Munoz-Martinez, F., Reyes-Darias, J. A., Duque, E., Matilla, M., Segura, A., Calvo, J. J., Jimenez-Sanchez, C., Krell, T. and Ramos, J. L. (2011). Bacterial chemotaxis towards aromatic hydrocarbons in Pseudomonas. Environ Microbiol 13(7): 1733-1744.
  16. Krell, T., Lacal, J., Munoz-Martinez, F., Reyes-Darias, J. A., Cadirci, B. H., Garcia-Fontana, C. and Ramos, J. L. (2011). Diversity at its best: bacterial taxis. Environ Microbiol 13(5): 1115-1124.
  17. Sanchez-Luque, F. J., Reyes-Darias, J. A., Puerta-Fernandez, E. and Berzal-Herranz, A. (2010). Inhibition of HIV-1 replication and dimerization interference by dual inhibitory RNAs. Molecules 15(7): 4757-4772.
  18. Marton, S., Reyes-Darias, J. A., Sanchez-Luque, F. J., Romero-Lopez, C. and Berzal-Herranz, A. (2010). In vitro and ex vivo selection procedures for identifying potentially therapeutic DNA and RNA molecules. Molecules 15(7): 4610-4638.
  19. Reyes-Darias, J. A., Sanchez-Luque, F. J. and Berzal-Herranz, A. (2008). Inhibition of HIV-1 replication by RNA-based strategies. Curr HIV Res 6(6): 500-514.

1 Protocol published
使用量化毛细管和梯度平板趋化性试验法进行细菌化学受体的识别和特性描述
Bacterial chemotaxis is a motility-based response that biases cell movement toward beneficial molecules, called attractants, and away from harmful molecules, also known as repellents. Since the species of the genus Pseudomonas are characterized by a ...
11 Protocols reviewed
Synchronized Real-time Measurement of Sec-mediated Protein Translocation
Authors:  Riti Gupta, Dmitri Toptygin and Christian M. Kaiser, date: 08/20/2021, view: 699, Q&A: 0

The Sec translocon, consisting of a heterotrimeric transmembrane channel (SecYEG) and an associated ATPase (SecA), catalyzes the export of unfolded proteins from the cytosol in bacteria. Kinetically resolving protein translocation at high resolution

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In vitro Glutamylation Inhibition of Ubiquitin Modification and Phosphoribosyl-Ubiquitin Ligation Mediated by Legionella pneumophila Effectors
Authors:  Alan G. Sulpizio, Marena E. Minelli and Yuxin Mao, date: 11/05/2020, view: 1165, Q&A: 0

Glutamylation is a posttranslational modification where the amino group of a free glutamate amino acid is conjugated to the carboxyl group of a glutamate side chain within a target protein. SidJ is a Legionella kinase-like protein that has recently

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